{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T06:08:34Z","timestamp":1790489314717,"version":"4.1.0"},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100011621","name":"Winship Cancer Institute","doi-asserted-by":"publisher","award":["00075389"],"award-info":[{"award-number":["00075389"]}],"id":[{"id":"10.13039\/100011621","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>While the importance of commensal microbes in vaginal health is well appreciated, little is known about the effects of gynecological cancer (GynCa) and radiation therapy (RT) on the vaginal microbiome (VM) of postmenopausal women.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We studied women with GynCa, pre\u2010 (N\u00a0=\u00a065) and post\u2010RT (N\u00a0=\u00a025) and a group of healthy controls (N\u00a0=\u00a067) by sequencing the V4 region of the 16S rRNA gene from vaginal swabs and compared the diversity and composition of VMs between the three groups accounting for potential confounding factors in multivariate analysis of variance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Comparisons of cancer vs healthy groups revealed that\n                      <jats:italic>Lactobacillus<\/jats:italic>\n                      and\n                      <jats:italic>Bifidobacterium<\/jats:italic>\n                      have significantly higher relative abundance in the healthy group, while the cancer group was enriched in 16 phylogroups associated with bacterial vaginosis (BV) and inflammation, including\n                      <jats:italic>Sneathia<\/jats:italic>\n                      ,\n                      <jats:italic>Prevotella<\/jats:italic>\n                      ,\n                      <jats:italic>Peptoniphilus<\/jats:italic>\n                      ,\n                      <jats:italic>Fusobacterium<\/jats:italic>\n                      ,\n                      <jats:italic>Anaerococcus<\/jats:italic>\n                      ,\n                      <jats:italic>Dialister<\/jats:italic>\n                      ,\n                      <jats:italic>Moryella,<\/jats:italic>\n                      and\n                      <jats:italic>Peptostreptococcus<\/jats:italic>\n                      . In our sample, RT affected the \u03b1\u2010diversity and correlated with higher abundance of typically rare VM species, including several members of the\n                      <jats:italic>Lacnospiraceae<\/jats:italic>\n                      family, a taxon previously linked to vaginal dysbiosis. In addition to cancer and treatment modalities, age and vaginal pH were identified as significant parameters that structure the VM.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This is among the first reports identifying VM changes among postmenopausal women with cancer. RT alone seems to affect several phylogroups (12 bacterial genera), while gynecological cancer and its treatment modalities are associated with even greater significant shifts in the vaginal microbiota including the enrichment of opportunistic bacterial pathogens, which warrants further attention.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.3027","type":"journal-article","created":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T11:38:04Z","timestamp":1585741084000},"page":"3714-3724","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Comparison of vaginal microbiota in gynecologic cancer patients pre\u2010 and post\u2010radiation therapy and healthy women"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4578-1918","authenticated-orcid":false,"given":"Despina","family":"Tsementzi","sequence":"first","affiliation":[{"name":"Nell Hodgson Woodruff School of Nursing Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Pena\u2010Gonzalez","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Georgia Institute of Technology Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbing","family":"Bai","sequence":"additional","affiliation":[{"name":"Nell Hodgson Woodruff School of Nursing Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Juan","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Bioinformatics Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pretesh","family":"Patel","sequence":"additional","affiliation":[{"name":"Radiation Oncology Winship Cancer Institute Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Shelton","sequence":"additional","affiliation":[{"name":"Radiation Oncology Winship Cancer Institute Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"Dolan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica","family":"Arluck","sequence":"additional","affiliation":[{"name":"Grady Memorial Hospital Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Namita","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lesley","family":"Conrad","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Scott","sequence":"additional","affiliation":[{"name":"Grady Memorial Hospital Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tony Y.","family":"Eng","sequence":"additional","affiliation":[{"name":"Radiation Oncology Winship Cancer Institute Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantinos T.","family":"Konstantinidis","sequence":"additional","affiliation":[{"name":"School of Biological Sciences Georgia Institute of Technology Atlanta GA USA"},{"name":"School of Civil &amp; Environmental Engineering Georgia Institute of Technology Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah W.","family":"Bruner","sequence":"additional","affiliation":[{"name":"Nell Hodgson Woodruff School of Nursing Emory University Atlanta GA USA"},{"name":"Radiation Oncology Winship Cancer Institute Emory University Atlanta GA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2019.103696"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajog.2018.11.1089"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1002611107"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3003605"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/GME.0b013e3182a4690b"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/2049-2618-2-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.maturitas.2016.05.015"},{"key":"e_1_2_8_9_1","unstructured":"CDC.Gynecologic Cancers [Internet].2019.https:\/\/www.cdc.gov\/cancer\/gynecologic\/index.htm. Accessed December 9 2019."},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/CCO.0000000000000215"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1080\/0284186X.2016.1275778"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0360-3016(03)00362-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/1471-0528.14631"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1097\/IGC.0000000000000999"},{"key":"e_1_2_8_15_1","unstructured":"HMP.Manual of Procedures for Human Microbiome Project Version 12.0 (dbGaP ID: phd003190)."},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01043-13"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.00579"},{"key":"e_1_2_8_18_1","unstructured":"OksanenJ BlanchetFG KindtR et al.vegan: Community Ecology Package [Internet].2013.http:\/\/cran.r\u2010project.org\/web\/packages\/vegan\/index.html. Accessed January 10 2014."},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2658"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2011-12-6-r60"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-S8-S4"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aad3369"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep16865"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.anaerobe.2017.05.012"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep38506"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.3389\/fphys.2015.00253"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00075-15"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2015.04.019"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.2147\/CMAR.S217362"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2018.00371"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17351-9"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-18-0233"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0080254"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00410-17"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41591-019-0600-6"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.3027","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.3027","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.3027","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.3027","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,5]],"date-time":"2023-09-05T22:02:12Z","timestamp":1693951332000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.3027"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4]]},"references-count":36,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/cam4.3027"],"URL":"https:\/\/doi.org\/10.1002\/cam4.3027","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4]]},"assertion":[{"value":"2020-03-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}