{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T08:01:22Z","timestamp":1771660882933,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"14","license":[{"start":{"date-parts":[[2020,5,27]],"date-time":"2020-05-27T00:00:00Z","timestamp":1590537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Hepatocellular carcinoma (HCC) is one of the leading causes of cancer\u2010related death worldwide. Numerous analyses have revealed the abnormal expression of long non\u2010coding RNAs (lncRNAs) in HCC cells. This study aims to explore biological functions of lncRNA TMPO\u2010AS1 (TMPO antisense RNA 1) in HCC cell proliferation, apoptosis, invasion and migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The gene expression in HCC tissues and cell lines were measured by qRT\u2010PCR. The role of TMPO\u2010AS1 in HCC was confirmed by CCK\u20108, colony formation, TUNEL, transwell and western blot as well as by in vivo experiments. RNA pull down and luciferase reporter assays were utilized to prove the binding relationship between TMPO\u2010AS1\/FOXK1 (forkhead box K1) andmiR\u2010329\u20103p. Rescue assays elucidated the regulatory effects of TMPO\u2010AS1\/miR\u2010329\u20103p\/FOXK1\/AKT\/mTOR pathway on cellular activities in HCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TMPO\u2010AS1was upregulated in HCC tissues and cells and its depletion inhibits HCC cell proliferation, invasion, migration, and EMT process as well as tumor growth. Furthermore, TMPO\u2010AS1 could bind with miR\u2010329\u20103p, which suppressed HCC cell proliferation. FOXK1 served as the target gene of miR\u2010329\u20103p and TMPO\u2010AS1 upregulated FOXK1 by sponging miR\u2010329\u20103p in HCC cells. Additionally, FOXK1 overexpression or miR\u2010329\u20103p inhibitor neutralized the repressing effects of TMPO\u2010AS1 knockdown on HCC development. Finally, it verified that TMPO\u2010AS1 could regulate AKT\/mTOR pathway via FOXK1 to promote HCC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TMPO\u2010AS1 contributes to HCC progression by sponging miR\u2010329\u20103p to activate FOXK1\u2010mediated AKT\/mTOR signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3046","type":"journal-article","created":{"date-parts":[[2020,5,28]],"date-time":"2020-05-28T05:35:30Z","timestamp":1590644130000},"page":"5235-5246","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["LncRNA TMPO\u2010AS1 promotes hepatocellular carcinoma cell proliferation, migration and invasion through sponging miR\u2010329\u20103p to stimulate FOXK1\u2010mediated AKT\/mTOR signaling pathway"],"prefix":"10.1002","volume":"9","author":[{"given":"Xiaobo","family":"Guo","sequence":"first","affiliation":[{"name":"Department of Hematology Xi'an Central Hospital 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