{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T18:02:13Z","timestamp":1792692133299,"version":"4.5.0"},"reference-count":33,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2020,5,5]],"date-time":"2020-05-05T00:00:00Z","timestamp":1588636800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7161012"],"award-info":[{"award-number":["7161012"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81741142"],"award-info":[{"award-number":["81741142"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Intravenous leiomyomatosis (IVL) is currently regarded as a special variant of the common uterine leiomyoma (LM). Though IVL shows a similar histological morphology to LM, IVL is characterized by unique intravenous growth patterns and low\u2010grade malignant potential, which are quite different from LM. There are currently few studies underlying the molecular alterations of IVL, though this information is important for understanding the pathogenesis of the disease, and for identifying potential biomarkers.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>We carried out a high\u2010throughput whole transcriptome sequencing of tumor and normal tissue samples from five IVL patients and five LM patients and compared the differentially expressed genes (DEGs) between IVL and leiomyoma. We performed multiple different enrichment and target analyses, and the expression of selected DEGs was validated using RT\u2010qPCR in formalin\u2010fixed samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our study identified substantial different genes and pathways between IVL and LM, and functional enrichment analyses found several important pathways, such as angiogenesis and antiapoptosis pathways, as well as important related genes, including\n                      <jats:italic>SH2D2A, VASH2, ADAM8, GATA2, TNF,<\/jats:italic>\n                      and the lncRNA\n                      <jats:italic>GATA6\u2010AS1<\/jats:italic>\n                      , as being significantly different between IVL and LM (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0024,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0195,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0212,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0435,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0401, and\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0246, respectively).\n                      <jats:italic>CXCL8, LIF, CDKN2A, BCL2A1, COL2A1, IGF1,<\/jats:italic>\n                      and\n                      <jats:italic>HMGA2<\/jats:italic>\n                      were also differently expressed between IVL and LM groups, but showed no statistical difference (\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.2409,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.1773,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.0596,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.2737,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.1553,\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.1045, and\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.1847, respectively) due to the large differences among individuals. Furthermore, RT\u2010qPCR results for five selected DEGs in IVL tissues and adjacent nontumor tissues were mainly consistent with our sequencing results.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our results indicated that IVL may be a solid entity that is unique and different from LM, proving consistent with previous studies. Furthermore, we identified DEGs, particularly within angiogenesis and antiapoptosis pathway\u2010related genes that may play crucial roles in the development and pathogenesis of IVL and may be potential specific biomarkers.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.3098","type":"journal-article","created":{"date-parts":[[2020,5,6]],"date-time":"2020-05-06T02:56:01Z","timestamp":1588733761000},"page":"4581-4592","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Intravenous leiomyomatosis is inclined to a solid entity different from uterine leiomyoma based on RNA\u2010seq analysis with RT\u2010qPCR validation"],"prefix":"10.1002","volume":"9","author":[{"given":"Wenze","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pathology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Science Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Heilongjiang Province Land Reclamation Headquarter General Hospital Harbin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gynecology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Science Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Haidian Maternal &amp; Children Health Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rujing","family":"Jia","sequence":"additional","affiliation":[{"name":"Accreditation Dept Five (Proficiency Testing Dept.) 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