{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:44:55Z","timestamp":1792683895247,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T00:00:00Z","timestamp":1593561600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004608","name":"Natural Science Foundation of Jiangsu Province","doi-asserted-by":"publisher","award":["BK20171181"],"award-info":[{"award-number":["BK20171181"]}],"id":[{"id":"10.13039\/501100004608","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Purpose<\/jats:title><jats:p>Anti\u2010CD19 chimeric antigen receptor T (CAR\u2010T) cell therapy has demonstrated remarkable efficacy for refractory and relapsed diffuse large B cell lymphoma (R\/R DLBCL). However, this therapy failed in nearly 25% patients mainly due to antigen loss. The authors performed a phase \u2161 trial by coadministration of anti\u2010CD19 and anti\u2010CD20 CAR\u2010T cells treatment for R\/R DLBCL and evaluated its efficacy and toxicity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Totally 21 patients with DLBCL were enrolled in this study. The patients were conditioned with fludarabine and cyclophosphamide before the infusion of anti\u2010CD19 and anti\u2010CD20 CAR\u2010T cells. Treatment response, toxicity, and persistence were monitored continuously.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Of the 21 patients received the treatment, the objective response rate (ORR) is 81.0% (95% confidence interval [CI], 58.1%\u201094.6%) with four cases of bulk (4\/5) and one case of testis involvement; 52.4% (95% CI, 29.8%\u201074.3%) had a complete response (CR). Peak levels of anti\u2010CD19 and anti\u2010CD20 CAR cells were associated with response (<jats:italic>P<\/jats:italic>\u00a0=\u00a0.007 and .002). Grade 3\u20104 cytokine release syndrome (CRS) and neurologic events occurred in 28.5% and 9.5% patients, respectively. Median overall survival (OS) and progression\u2010free survival (PFS) were 8.1 and 5.0\u00a0months, respectively. The maximum standard uptake value (SUVmax) of CD4\/CD8 ratio before and after infusion were associated with responses, and the total lesion glycolysis (TLG) before infusion correlates with cytokines level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Coadministration of anti\u2010CD19 and CD20 CAR\u2010T cells therapy for DLBCL is feasible with manageable toxicity. Cytokine markers are related to toxicity and SUVmax could predict efficacy. This trial was registered at <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" xlink:href=\"http:\/\/www.clinicaltrials.gov\">www.clinicaltrials.gov<\/jats:ext-link> as NCT03207178.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3259","type":"journal-article","created":{"date-parts":[[2020,7,1]],"date-time":"2020-07-01T11:50:24Z","timestamp":1593604224000},"page":"5827-5838","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":69,"title":["Phase II trial of co\u2010administration of CD19\u2010 and CD20\u2010targeted chimeric antigen receptor T cells for relapsed and refractory diffuse large B cell lymphoma"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1170-7074","authenticated-orcid":false,"given":"Wei","family":"Sang","sequence":"first","affiliation":[{"name":"Department of Hematology the Affiliated Hospital of Xuzhou Medical University Xuzhou 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