{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:38:58Z","timestamp":1788961138450,"version":"build-2803163510"},"reference-count":32,"publisher":"Wiley","issue":"17","license":[{"start":{"date-parts":[[2020,7,7]],"date-time":"2020-07-07T00:00:00Z","timestamp":1594080000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["18411965300"],"award-info":[{"award-number":["18411965300"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Current evidence on diagnostic value of aneuploidy with DNA image cytometry (ICM) using brushings for oral potentially malignant disorders (OPMD) is limited by sample size and inconsistent classification criteria of aneuploidy. This study aimed to explore the optimal cut\u2010off values of DNA content and evaluate the diagnostic accuracy of DNA\u2010ICM for detecting dysplasia and\/or carcinoma in OPMD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A total of 401 consecutive patients with OPMD were enrolled in this prospective diagnostic study. Brushing and biopsy sample form each patient was processed by DNA\u2010ICM and histological examination respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When the optimal cut\u2010off of at least one aneuploid cell with DNA index (DI) \u22652.3, the area under the curves (AUC) was 0.735 and positive predictive value was 92.7% for detecting dysplasia within OPMD. When the optimal cut\u2010off of at least one aneuploid cell with DI\u00a0\u2265\u00a03.5, the AUC was 0.851 and negative predictive values was 96.8% for detecting carcinoma within OPMD. Importantly, multivariate analysis revealed that aneuploidy with DI\u00a0\u2265\u00a02.3 in OPMD was significantly associated with dysplasia risk (adjusted OR, 5.52; 95%CI, 2.90\u201010.51; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001), and aneuploidy with DI\u00a0\u2265\u00a03.5 in OPMD was strongly associated with malignant risk (adjusted OR, 21.05; 95%CI, 9.34\u201047.41; <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This largest\u2010scale diagnostic study optimized the criteria of aneuploidy cytology for noninvasive detection of oral dysplasia and carcinoma within OPMD. DNA aneuploidy in OPMD was an independent marker that strongly associated with oral dysplasia\/carcinoma. Our findings suggest that DNA\u2010ICM may serve as a useful noninvasive adjunctive tool for oral cancer and OPMD screening.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3293","type":"journal-article","created":{"date-parts":[[2020,7,8]],"date-time":"2020-07-08T06:35:07Z","timestamp":1594190107000},"page":"6411-6420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["DNA aneuploidy with image cytometry for detecting dysplasia and carcinoma in oral potentially malignant disorders: A prospective diagnostic study"],"prefix":"10.1002","volume":"9","author":[{"given":"Chenxi","family":"Li","sequence":"first","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yiwen","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuemin","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology &amp; Shanghai Research Institute of Stomatology Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4841-8356","authenticated-orcid":false,"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial\u2010Head and Neck Oncology Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjun","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Ninth People's Hospital College of Stomatology Shanghai Jiao Tong University School of Medicine National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology &amp; 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