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However, patients do not always derive benefits from genomic testing. Here, we performed targeted amplicon exome sequencing\u2010based panel tests, including 160 cancer\u2010related genes (PleSSision\u2010160), on 88 malignant ovarian tumors (high\u2010grade serous carcinoma, 27; endometrioid carcinoma, 15; clear cell carcinoma, 30; mucinous carcinoma, 6; undifferentiated carcinoma, 4; and others, 6 (immature teratoma, 1; carcinosarcoma, 3; squamous cell carcinoma, 1; and mixed, 1)), to assess treatment strategies and useful biomarkers for malignant ovarian tumors. Overall, actionable gene variants were found in 90.9%, and druggable gene variants were found in 40.9% of the cases. Actionable<jats:italic>BRCA1<\/jats:italic>and<jats:italic>BRCA2<\/jats:italic>variants were found in 4.5% of each of the cases.<jats:italic>ERBB2<\/jats:italic>amplification was found in 33.3% of mucinous carcinoma cases. Druggable hypermutation\/ultramutation (tumor mutation burden\u00a0\u2265\u00a010 SNVs\/Mbp) was found in 7.4% of high\u2010grade serous carcinoma, 46.7% of endometrioid carcinoma, 10% of clear cell carcinoma, 0% of mucinous carcinoma, 25% of undifferentiated carcinoma, and 33.3% of the other cancer cases. Copy number alterations were significantly higher in high\u2010grade serous carcinoma (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.005) than in other histologic subtypes; some clear cell carcinoma showed high copy number alterations that were correlated with advanced stage (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05) and worse survival (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.01). A high count of copy number alteration was associated with worse survival in all malignant ovarian tumors (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05). Our study shows that targeted agents can be detected in approximately 40% of malignant ovarian tumors via multigene panel testing, and copy number alteration count can be a useful marker to help assess risks in malignant ovarian tumor patients.<\/jats:p>","DOI":"10.1002\/cam4.3383","type":"journal-article","created":{"date-parts":[[2020,8,28]],"date-time":"2020-08-28T05:23:11Z","timestamp":1598592191000},"page":"7407-7417","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["Clinical implications of next\u2010generation sequencing\u2010based panel tests for malignant ovarian tumors"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5619-5148","authenticated-orcid":false,"given":"Keiko","family":"Saotome","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Keio University School of Medicine Tokyo 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