{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T18:56:53Z","timestamp":1785783413460,"version":"3.56.0"},"reference-count":54,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T00:00:00Z","timestamp":1602547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2020,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>In patients with squamous cell carcinoma of the oral tongue (OTSCC), current tumor node metastasis staging system fails to identify at\u2010risk patients associated with early relapse and poor prognosis despite complete surgical resection. Given the importance of tumor\u2010infiltrating lymphocytes (TILs) in the development of cancers, here we investigated the prognostic significance of the immune phenotype in OTSCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Hematoxylin\u2010eosin stained sections of OTSCCs from 211 patients were evaluated. Cancers were classified as (a) immune\u2010inflamed when TILs were found next to tumor cell nests; (b) immune\u2010excluded when TILs were found in the stroma, outside the tumor; and (c) immune\u2010desert for tumors lacking lymphocyte infiltrate. The prognostic significance of these immune phenotypes classes was investigated. Data were further validated on an independent cohort from The Cancer Genome Atlas database.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Immune\u2010desert phenotype was the least represented group of OTSCCs in our cohort (11.8%) and served as an independent prognostic factor. Patients with immune\u2010desert tumors exhibited worse disease\u2010specific survival (HR\u00a0=\u00a02.673; [CI: 95% 1.497\u20104.773];\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.001), overall survival (HR\u00a0=\u00a02.591; [CI: 95% 1.468\u20104.572];\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.001), and disease\u2010free survival (HR\u00a0=\u00a02.313; [CI: 95% 1.118\u20104.786];\n                      <jats:italic>P<\/jats:italic>\n                      \u00a0=\u00a0.024) at multivariate analysis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>We identified a specific subgroup of OTSCCs with poor prognosis. Tumor\u2010infiltrating lymphocytes density and localization could serve as an integrative parameter to the current staging system and inform the selection of most appropriate treatments. In particular, the tumor immune phenotype could improve the stratification of patients with more aggressive disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.3440","type":"journal-article","created":{"date-parts":[[2020,10,13]],"date-time":"2020-10-13T10:27:22Z","timestamp":1602584842000},"page":"8333-8344","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["The immune phenotype of tongue squamous cell carcinoma predicts early relapse and poor prognosis"],"prefix":"10.1002","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-4414","authenticated-orcid":false,"given":"Giuseppe","family":"Troiano","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corrado","family":"Rubini","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Public Health Marche Polytechnic University Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucrezia","family":"Togni","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences Marche Polytechnic University Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5080-5921","authenticated-orcid":false,"given":"Vito Carlo Alberto","family":"Caponio","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khrystyna","family":"Zhurakivska","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea","family":"Santarelli","sequence":"additional","affiliation":[{"name":"Department of Clinical Specialistic and Dental Sciences Marche Polytechnic University Ancona Italy"},{"name":"Dentistry Clinic National Institute of Health and Science of Aging INRCA Ancona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicola","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Vic. 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