{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:02:34Z","timestamp":1790812954004,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T00:00:00Z","timestamp":1607212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81873726"],"award-info":[{"award-number":["81873726"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Lidocaine, one of the most commonly used local anesthetics during surgery, has been reported to suppress cancer cell growth via blocking voltage\u2010gated sodium channels (VGSCs). VGSC 1.5 (Na<jats:sub>V<\/jats:sub>1.5) is highly expressed in invasive cancers including ovarian cancer. This study aims to investigate whether lidocaine inhibits the malignancy of ovarian cancer through Na<jats:sub>V<\/jats:sub>1.5 blockage. Human ovarian cancer, its metastatic cancer and normal ovarian tissues were probed with anti\u2010Na<jats:sub>V<\/jats:sub>1.5 antibody <jats:italic>in situ<\/jats:italic>. Human ovarian cancer A2780 and SKOV3 cells were cultured and their growth, epithelial\u2010mesenchymal transition (EMT), migration, and invasion in the presence or absence of lidocaine together with underlying molecular mechanisms were assessed. Murine syngeneic ovarian cancer (ID8) model was also used to determine the chemotherapeutic efficiency of cisplatin in combination with lidocaine. The high level of Na<jats:sub>V<\/jats:sub>1.5 expression was found in human ovarian cancer and even higher in its metastatic cancer but not in normal ovarian tissues. Lidocaine decreased the growth, EMT, migration, and invasion of human ovarian cancer A2780 and SKOV3 cells. Lidocaine enhanced the chemotherapeutic efficiency of cisplatin in both ovarian cancer cell cultures and a murine ovarian metastatic model. Furthermore, a downregulation of Na<jats:sub>V<\/jats:sub>1.5 by siRNA transfection, or FAK inhibitor application, inhibited the malignant properties of SKOV3 cells through inactivating FAK\/Paxillin signaling pathway. Our data may indicate that lidocaine suppresses the metastasis of ovarian cancer and sensitizes cisplatin through blocking Na<jats:sub>V<\/jats:sub>1.5\u2010mediated EMT and FAK\/paxillin signaling pathway. The translational value of lidocaine local application as an ovarian cancer adjuvant treatment warrants further study.<\/jats:p>","DOI":"10.1002\/cam4.3621","type":"journal-article","created":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T12:23:00Z","timestamp":1607257380000},"page":"337-349","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["Lidocaine inhibits the metastatic potential of ovarian cancer by blocking Na<sub>V<\/sub>1.5\u2010mediated EMT and FAK\/Paxillin signaling pathway"],"prefix":"10.1002","volume":"10","author":[{"given":"Chang","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Anesthesiology Peking University Third Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Dalian Medical University Dalian 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