{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,13]],"date-time":"2025-05-13T14:39:20Z","timestamp":1747147160281,"version":"3.40.5"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T00:00:00Z","timestamp":1607212800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2021,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Human epidermal growth factor receptor 2\u2010in situ hybridization (HER2\u2010ISH) is widely approved for diagnostic, prognostic biomarker testing of formalin\u2010fixed paraffin\u2010embedded tissue blocks. However, cytologic ISH analysis has a potential advantage in tumor samples such as pleural effusion and ascites that are difficult to obtain the histological specimens. Our aim was to evaluate the clinical reliability of a novel rapid cytologic HER2 fluorescence ISH protocol (rapid\u2010CytoFISH).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Using a new device, we applied a high\u2010voltage\/frequency, noncontact alternating current electric field to tissue imprints and needle rinses, which mixed the probe within microdroplets as the voltage was switched on and off (AC mixing). Cytologic samples (n\u00a0=\u00a0143) were collected from patients with immunohistochemically identified HER2 breast cancers. The specimens were then tested using standard dual\u2010color ISH using formalin\u2010fixed paraffin\u2010embedded tissue (FFPE\u2010tissue DISH) for HER2\u2010targeted therapies, CytoFISH, and rapid\u2010CytoFISH (completed within 4\u00a0h).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All 143 collected cytologic specimens (50 imprinted cytology specimens from resected tumors and 93 liquid\u2010based cytology specimens from needle rinses) were suitable for FISH analysis. The HER2\/chromosome enumeration probe (CEP) 17 ratios did not significantly differ between FFPE\u2010tissue DISH and either CytoFISH protocol. Based on HER2 scoring criteria, we found 95.1% agreement between FFPE\u2010tissue DISH and CytoFISH (Cohen's kappa coefficient\u00a0=\u00a00.771 and 95% confidence interval (CI): 0.614\u20130.927).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CytoFISH could potentially serve as a clinical tool for prompt determination of HER2 status in breast cancer cytology. Rapid\u2010CytoFISH with AC mixing will enable cancer diagnoses and HER2 status to be determined on the same day a patient comes to a clinic or hospital.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3626","type":"journal-article","created":{"date-parts":[[2020,12,6]],"date-time":"2020-12-06T12:40:00Z","timestamp":1607258400000},"page":"586-594","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Rapid HER2 cytologic fluorescence in situ hybridization for breast cancer using noncontact alternating current electric field mixing"],"prefix":"10.1002","volume":"10","author":[{"given":"Shin\u2010nosuke","family":"Watanabe","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery Akita University Graduate School of Medicine Akita 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