{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T04:00:05Z","timestamp":1778558405996,"version":"3.51.4"},"reference-count":30,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T00:00:00Z","timestamp":1608595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005150","name":"Chinese Academy of Medical Sciences","doi-asserted-by":"publisher","award":["2016\u2010I2M\u20101\u2010002"],"award-info":[{"award-number":["2016\u2010I2M\u20101\u2010002"]}],"id":[{"id":"10.13039\/501100005150","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81602162"],"award-info":[{"award-number":["81602162"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Pancreatic cancer (PC) is one of the most lethal malignancies with an increasing death rate over the years. We performed targeted sequencing and survival analyses on 90 Chinese pancreatic cancer patients, hoping to identify genomic biomarkers associated with clinical outcomes and therapeutic options.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Genomic DNA was extracted from formalin\u2010fixed paraffin\u2010embedded (FFPE) tissue specimens of 90 pancreatic cancer patients and sequenced. The associations with clinicopathological factors were analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>High prevalence of driver mutations in <jats:italic>KRAS<\/jats:italic>, <jats:italic>TP53<\/jats:italic>, <jats:italic>CDKN2A<\/jats:italic>, <jats:italic>SMAD4<\/jats:italic>, and <jats:italic>ARID1A<\/jats:italic> genes were found. Most mutated genes in PC belonged to cell cycle and DNA damage repair pathways. Tumors that arise from the pancreas\u2019 body and tail (BT tumors) displayed a higher ratio of mutated <jats:italic>KRAS<\/jats:italic> and <jats:italic>TP53<\/jats:italic> than those that arise from the pancreas\u2019 head and neck (HN tumors), who showed less diverse <jats:italic>KRAS<\/jats:italic> subtypes. Patients with a <jats:italic>KRAS<\/jats:italic> p.G12R mutated tumor tended to have a prolonged disease\u2010free survival (DFS) and overall survival (OS) than other <jats:italic>KRAS<\/jats:italic> subtypes. Those with an altered <jats:italic>ARID1A<\/jats:italic> gene and more than two mutated driver genes tended to have a shorter DFS and OS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HN and BT tumors of the pancreas displayed different mutational profiles, which had prognostic significances and indicated different potential therapeutic options.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3679","type":"journal-article","created":{"date-parts":[[2020,12,22]],"date-time":"2020-12-22T20:32:40Z","timestamp":1608669160000},"page":"933-943","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Genetic aberrations in Chinese pancreatic cancer patients and their association with anatomic location and disease outcomes"],"prefix":"10.1002","volume":"10","author":[{"given":"Junliang","family":"Lu","sequence":"first","affiliation":[{"name":"Department of Pathology Molecular Pathology Research Center Peking Union Medical College Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ruoying","family":"Yu","sequence":"additional","affiliation":[{"name":"Translational Medicine Research Institute Geneseeq Technology Inc Toronto ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rui","family":"Liu","sequence":"additional","affiliation":[{"name":"Translational Medicine Research Institute Geneseeq Technology Inc Toronto ON Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaolong","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Pathology Beijing Chaoyang Hospital Capital Medical University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Pathology Molecular Pathology Research Center Peking Union Medical College Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Molecular Pathology Research Center Peking Union Medical College Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huanwen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathology Molecular Pathology Research Center Peking Union Medical College Hospital Chinese Academy of Medical Sciences &amp; Peking Union Medical College Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhiwen","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology Molecular Pathology Research Center Peking Union Medical College Hospital Chinese Academy 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