{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T21:25:04Z","timestamp":1785014704514,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2021,1,15]],"date-time":"2021-01-15T00:00:00Z","timestamp":1610668800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012353","name":"Deutschen Konsortium f\u00fcr Translationale Krebsforschung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100012353","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008672","name":"Wilhelm Sander-Stiftung","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008672","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["CRC 1066\u2010B3)"],"award-info":[{"award-number":["CRC 1066\u2010B3)"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2021,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Malignant melanoma is an immunogenic skin cancer with an increasing global incidence. Advanced stages of melanoma have poor prognoses. Currently, there are no reliable parameters to predict a patient's response to immune checkpoint inhibitor (ICI) therapy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study highlights the relevance of a distinct immune signature in the blood for response to ICI therapy and overall survival (OS). Therefore, the immune cell composition in the peripheral blood of 45 melanoma patients prior to ICI therapy was analyzed by flow cytometry and complete blood count.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Responders to ICI therapy displayed an abundance of proliferating CD4<jats:sup>+<\/jats:sup> T cells, an increased lymphocyte\u2010to\u2010monocyte ratio, a low platelet\u2010to\u2010lymphocyte ratio, low levels of CTLA\u20104<jats:sup>+<\/jats:sup> Treg, and (arginase 1<jats:sup>+<\/jats:sup>) polymorphonuclear myeloid\u2010derived suppressor cells (PMN\u2010MDSC). Nevertheless, non\u2010responders with similar immune cell compositions also benefited from therapy displaying increased long\u2010term OS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrated that the observed immune signature in the peripheral blood of melanoma patients prior to treatment could identify responders as well as non\u2010responders that benefit from ICI immunotherapies.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.3710","type":"journal-article","created":{"date-parts":[[2021,1,16]],"date-time":"2021-01-16T00:04:28Z","timestamp":1610755468000},"page":"1562-1575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Immune signature as predictive marker for response to checkpoint inhibitor immunotherapy and overall survival in melanoma"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9419-6782","authenticated-orcid":false,"given":"Franziska K.","family":"Krebs","sequence":"first","affiliation":[{"name":"Department of Dermatology University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"},{"name":"German Cancer Consortium (DKTK) Partner Site Mainz\/Frankfurt am Main Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily R.","family":"Trzeciak","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center of the Johannes Gutenberg\u2010University Mainz Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophia","family":"Zimmer","sequence":"additional","affiliation":[{"name":"Department of Dermatology University Medical Center of the Johannes Gutenberg\u2010University Mainz 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