{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:22:40Z","timestamp":1790695360773,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T00:00:00Z","timestamp":1423008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"EuroSARC 287"},{"name":"EuroSarc FP7-278472"},{"name":"Labex DevweCan ANR-10-LABX-0061"},{"name":"LYRIC INCA DHOS 4664"},{"name":"PHRC IC2007-09"},{"name":"Saone et Loire Committee of the French National League against Cancer"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2015,4]]},"abstract":"Abstract<\/jats:title>\n \n New protocols based on\n ALK<\/jats:styled-content>\n \u2010targeted therapy by crizotinib or other\n ALK<\/jats:styled-content>\n \u2010targeting molecules have opened for the treatment of patients with neuroblastoma (\n NB<\/jats:styled-content>\n ) if their tumors showed mutation and\/or amplification of the\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n gene. However, tumor samples are not always available for analysis of\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n mutational status in particular at relapse. Here, we evaluated the\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n mutational status of\n NB<\/jats:styled-content>\n samples by analysis of circulating\n DNA<\/jats:styled-content>\n , using the droplet digital\n PCR<\/jats:styled-content>\n (dd\n PCR<\/jats:styled-content>\n ) system. dd\n PCR<\/jats:styled-content>\n assays was developed for the detection of\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n mutations at F1174 and R1275 hotspots found in\n NB<\/jats:styled-content>\n tumors and was applied for the analysis of circulating\n DNA<\/jats:styled-content>\n obtained from 200\u00a0\n \u03bc<\/jats:italic>\n L of serum or plasma samples collected from 114 patients with\n NB<\/jats:styled-content>\n . The mutations F1174L (exon 23 position 3520, T>C and position 3522, C>A) and the mutation R1275Q (exon 25 position 3824, G>A) were detected in circulating\n DNA<\/jats:styled-content>\n . The sensitivity of our test was 100%, 85%, and 92%, respectively, and the specificity was 100%, 91%, and 98%, respectively. In conclusion, the assay that we have developed offers a reliable, noninvasive blood test to assess\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n mutational status at F1174 and R1275 hotspots and should help clinicians to identify patients showing an\n \n ALK<\/jats:styled-content>\n <\/jats:italic>\n mutation in particular when no tumor tissue is available.\n <\/jats:p>","DOI":"10.1002\/cam4.414","type":"journal-article","created":{"date-parts":[[2015,2,4]],"date-time":"2015-02-04T21:22:48Z","timestamp":1423084968000},"page":"540-550","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":75,"title":["Detection of tumor\n \n ALK<\/scp>\n <\/i>\n status in neuroblastoma patients using peripheral blood"],"prefix":"10.1002","volume":"4","author":[{"given":"Val\u00e9rie","family":"Combaret","sequence":"first","affiliation":[{"name":"Centre L\u00e9on\u2010B\u00e9rard Laboratoire de Recherche Translationnelle 28 rue Laennec 69373 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