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Mean survival time was 59.6\u00a0days for the mice in the HM86\u00a0group and significantly shorter (43.7\u00a0days) for HM19\u2010injected animals (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). In the HM86\u00a0group, the first detectable metastasis was located in the cerebellum in 15\/55 (29%) mice compared with none in the HM19\u00a0group (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001). At sacrifice, cerebellar metastases were found in 34\/55 (63%) HM86\u2010injected mice compared with 1\/53 (2%) in the HM19\u2010injected (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.001) mice. At that time, all mice in both groups had detectable metastases in the cerebrum. Comparing macroscopic and histologic appearances of the brain metastases with their clinical counterparts, the cell line\u2010based tumors had kept their original morphologic characteristics.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present work demonstrates that human brain\u2010metastatic melanoma cells injected intracardially in mice had retained inherent characteristics also in reproducing interaction with subtle microenvironmental brain tissue compartment\u2010specific features. The models offer new possibilities for investigating tumor\u2010 and host\u2010associated factors involved in determining tissue specificity of brain metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.4334","type":"journal-article","created":{"date-parts":[[2021,10,8]],"date-time":"2021-10-08T14:53:22Z","timestamp":1633704802000},"page":"8604-8613","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Novel human melanoma brain metastasis models in athymic nude fox1<sup>nu<\/sup> mice: Site\u2010specific metastasis patterns reflecting their clinical origin"],"prefix":"10.1002","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4169-5801","authenticated-orcid":false,"given":"Henrik A.","family":"Svendsen","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine Faculty of Medicine University of Oslo Oslo Norway"},{"name":"Department of Neurosurgery Oslo University Hospital Oslo 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