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HGSOCs often metastasize to the peritoneal cavity, while fluid cytology examination could identify such metastases. This retrospective study aimed to identify potential biomarker discrepancies between paired HGSOC primary tissues and metastatic peritoneal fluid cytology samples, processed as cell blocks (CBs).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four pairs of formalin\u2010fixed, paraffin\u2010embedded primary tissues and metastatic CBs from an equal number of treatment\u2010na\u00efve patients were used, and immunohistochemistry (IHC) for epidermal growth factor receptor (EGFR), human epidermal growth factor receptor, programmed cell death\u20101 ligand 1 (PD\u2010L1), and CD147 was applied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>13\/24 pairs showed discordant EGFR IHC results; in all these 13 patients, EGFR was positive (\u22651+ membranous staining intensity found in at least 10% of the cancer cells) in the peritoneal, yet negative in the primary tissue samples. Notably, EGFR IHC was positive in 15\/24 of the metastatic, whereas in just 2\/24 of the primary HGSOC samples (p\u00a0&lt;\u00a00.001). Although most PD\u2010L1 results were concordant, 5\/24 and 6\/24 pairs exhibited discordant results when stained with the E1L3N and 22C3 clones, respectively. Lastly, CD147 overexpression was found more often in the metastatic rather than the matched primary HGSOCs stained with CD147, though the difference was not significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Cytology from effusions could be considered for biomarker testing when present, even when tissue from the primary cancer is also available and adequately cellular, as it could provide additional information of potential clinical significance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.4600","type":"journal-article","created":{"date-parts":[[2022,2,25]],"date-time":"2022-02-25T11:10:15Z","timestamp":1645787415000},"page":"2193-2203","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Biomarkers expression among paired serous ovarian cancer primary lesions and their peritoneal cavity metastases in treatment\u2010na\u00efve patients: A single\u2010center 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