{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T19:20:00Z","timestamp":1772824800717,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T00:00:00Z","timestamp":1428883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["26290042"],"award-info":[{"award-number":["26290042"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Science, Sports, Culture and Technology of Japan","doi-asserted-by":"crossref","award":["24112005"],"award-info":[{"award-number":["24112005"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministry of Health, Labour and Welfare of Japan","award":["H26-Kakushinntekigan-ippann-082"],"award-info":[{"award-number":["H26-Kakushinntekigan-ippann-082"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2015,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Exon 2 of <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>12, a subunit of the transcriptional mediator complex, has been frequently mutated in uterine leiomyomas and breast fibroadenomas; however, it has been rarely mutated in other tumors. Although the mutations were also found in uterine leiomyosarcomas, the frequency was significantly lower than in uterine leiomyomas. Here, we examined the <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>12 mutation in phyllodes tumors, another biphasic tumor with epithelial and stromal components related to breast fibroadenomas. Mutations in <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>12 exon 2 were analyzed in nine fibroadenomas and eleven phyllodes tumors via Sanger sequencing. A panel of cancer\u2010 and sarcoma\u2010related genes was also analyzed using Ion Torrent next\u2010generation sequencing. Six mutations in fibroadenomas, including those previously reported (6\/9, 67%), and five mutations in phyllodes tumors (5\/11, 45%) were observed. Three mutations in the phyllodes tumors were missense mutations at Gly44, which is common in uterine leiomyomas and breast fibroadenomas. In addition, two deletion mutations (in\u2010frame c.133_144del12 and loss of splice acceptor c.100\u201068_137del106) were observed in the phyllodes tumors. No other recurrent mutation was observed with next\u2010generation sequencing. Frequent mutations in <jats:styled-content style=\"fixed-case\">MED<\/jats:styled-content>12 exon 2 in the phyllodes tumors suggest that it may share genetic etiology with uterine leiomyoma, a subgroup of uterine leiomyosarcomas and breast fibroadenoma.<\/jats:p>","DOI":"10.1002\/cam4.462","type":"journal-article","created":{"date-parts":[[2015,4,13]],"date-time":"2015-04-13T05:45:32Z","timestamp":1428903932000},"page":"1117-1121","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":45,"title":["MED12 exon 2 mutations in phyllodes tumors of the breast"],"prefix":"10.1002","volume":"4","author":[{"given":"Satoi","family":"Nagasawa","sequence":"first","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"},{"name":"Division of Breast and Endocrine Surgery Department of Surgery St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ichiro","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Pathology St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takayo","family":"Fukuda","sequence":"additional","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenwen","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryosuke","family":"Hayami","sequence":"additional","affiliation":[{"name":"Division of Breast and Endocrine Surgery Department of Surgery St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasuyuki","family":"Kojima","sequence":"additional","affiliation":[{"name":"Division of Breast and Endocrine Surgery Department of Surgery St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ko\u2010ichiro","family":"Tsugawa","sequence":"additional","affiliation":[{"name":"Division of Breast and Endocrine Surgery Department of Surgery St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomohiko","family":"Ohta","sequence":"additional","affiliation":[{"name":"Department of Translational Oncology St. Marianna University Graduate School of Medicine Kawasaki 216\u20108511 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2015,4,13]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1309\/U6DVBFM81MLJC1FN"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjsurg.2006.04.007"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-7404(03)00005-7"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(93)90362-T"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1767009"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tibs.2010.02.004"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208930"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2279"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.2953"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.428"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27610"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1302736"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0033251"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-014-0938-x"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/molehr\/gau055"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.01.006"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12039"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040015"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.203"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22029"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.243"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng.3037"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2014.03.047"},{"key":"e_1_2_6_25_1","first-page":"129","article-title":"Somatic 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