{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:18:10Z","timestamp":1790882290148,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"24","license":[{"start":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T00:00:00Z","timestamp":1652918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Stage IV colorectal cancer patients with liver metastasis represent a special group of CRC patients with poor prognosis. The prognostic factors have not been investigated for stage IV CRC patients undergoing primary cancer resection but not candidates for metastasis resection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ninety\u2010nine stage IV CRC patients who underwent primary cancer resection without metastasis resection were retrospectively recruited. Both whole\u2010exome sequencing (WES) and RNA\u2010seq were performed with frozen primary cancer tissues, using para\u2010cancerous normal tissues as the control. Valid data were obtained from 78 patients for WES and 84 patients for RNA\u2010seq. Univariate, multivariate Cox analyses were performed and Nomogram model was established to predict patient prognosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The correlation between patient prognosis and clinicopathological factors, mutational status, or mRNA level changes was examined. Univariate (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.0007) and subsequent multivariate analyses on clinicopathological factors showed that location (left or right) was the only independent risk factor for patient prognosis (HR\u00a0=\u00a03.63; 95% CI: 1.56\u20138.40, <jats:italic>p\u00a0=\u00a00<\/jats:italic>.003), while T, N, M staging, gender, race, location (rectum or colon), and pathological types were not stratifying factors. The mutational status of APC, TP53, KRAS, TTN, SYNE1, SMAD4, PIK3CA, RYR2, and BRAF did not show significant stratification in patient prognosis. RNA\u2010seq showed that genes related to membrane function, ion channels, transporters, or receptors were among those with significant mRNA level alterations. Univariate analysis identified 97 genes with significantly altered mRNA levels, while NEUROD1, FGF18, SFTA2, PLAC1, SAA2, DSCAML1, and OTOP3 were significant in multivariate analysis. A risk model was established to stratify the prognosis of stage IV CRC patients. A Nomogram model was established with these genes to predict individual patient prognosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A panel of eight genes with significant mRNA level alterations was capable of predicting the prognosis and risk of the specific patient group. Future prospective study is needed to validate the model.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.4824","type":"journal-article","created":{"date-parts":[[2022,5,19]],"date-time":"2022-05-19T09:26:01Z","timestamp":1652952361000},"page":"4900-4912","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Prognosis prediction of stage <scp>IV<\/scp> colorectal cancer patients by <scp>mRNA<\/scp> transcriptional profile"],"prefix":"10.1002","volume":"11","author":[{"given":"Bian","family":"Wu","sequence":"first","affiliation":[{"name":"Department of General Surgery II the First People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinwei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of General Surgery II the First People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiwei","family":"Qin","sequence":"additional","affiliation":[{"name":"Department of General Surgery Wenshan people's Hospital of Yunnan Province  Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongping","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Anus and Intestine Surgery Qujing Hospital of Traditional Chinese Medicine  Qujing Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingyi","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Reproductive Medicine the First People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2796-1309","authenticated-orcid":false,"given":"Yun","family":"Shang","sequence":"additional","affiliation":[{"name":"Department of General Surgery II the First People's Hospital of Yunnan Province  Kunming Yunnan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,5,19]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(19)32319-0"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2017.02.002"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdw235"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2019.5014"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2017.7011"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2019.05.004"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2019.06.038"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.esmoop.2021.100145"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70330-4"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clcc.2013.04.006"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.25578"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.soc.2017.11.010"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-8049(11)70148-4"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10151-018-1820-3"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2017.0038"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-019-07209-x"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdv494"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(13)61649-9"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.24774"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41575-019-0189-8"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.32489"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdx401"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ctrv.2019.04.003"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdz134"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1055-9965.EPI-20-0694"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.6004\/jnccn.2020.7680"},{"issue":"5","key":"e_1_2_12_28_1","first-page":"259","article-title":"Transcriptome analysis of CD133\u2010positive stem cells and prognostic value of survivin in colorectal cancer","volume":"11","author":"Kim ST","year":"2014","journal-title":"Cancer Genomics Proteomics"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/iub.1692"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2020.145072"},{"key":"e_1_2_12_31_1","doi-asserted-by":"publisher","DOI":"10.1042\/BSR20201725"},{"key":"e_1_2_12_32_1","doi-asserted-by":"publisher","DOI":"10.7150\/jca.38796"},{"key":"e_1_2_12_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-018-5193-9"},{"key":"e_1_2_12_34_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.14175"},{"key":"e_1_2_12_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2020.02.040"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.4824","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.4824","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.4824","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,20]],"date-time":"2023-08-20T11:12:24Z","timestamp":1692529944000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.4824"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,19]]},"references-count":34,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2022,12]]}},"alternative-id":["10.1002\/cam4.4824"],"URL":"https:\/\/doi.org\/10.1002\/cam4.4824","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,19]]},"assertion":[{"value":"2021-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-19","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}