{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T04:08:16Z","timestamp":1747714096357,"version":"3.40.5"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T00:00:00Z","timestamp":1661644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Malignant pleural mesothelioma (MPM) is a rare and highly malignant thoracic tumor. Although alternative splicing (AS) is associated with tumor prognosis, the prognostic significance of AS in MPM is unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Transcriptomic data, clinical information, and splicing percentage values for MPM were obtained from The Cancer Genome Atlas (TCGA) and TCGA SpliceSeq databases. Least absolute shrinkage and selection operator (LASSO) regression and multivariate Cox analyses were performed to establish a model affecting the prognosis of MPM. Survival and ROC analyses were used to test the effects of the prognostic model. LASSO\/multivariate Cox analysis was used to construct the MPM prognostic splicing factor (SF) model. The SF\u2013AS interaction network was analyzed using Spearman correlation and visualized using Cytoscape. The association between the MPM prognostic SF model and drug sensitivity to chemotherapeutic agents such as cisplatin was analyzed using pRRophetic.R.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The LASSO\/multivariate Cox analysis identified 41 AS events and 2 SFs that were mostly associated with survival. Nine prognostic prediction models (i.e., seven types of AS model, total AS model, and SF model) were developed. An MPM prognostic SF\u2013AS regulatory network was subsequently constructed with decreased drug sensitivity in the SF model high\u2010risk group (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.025).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study provides the first comprehensive analysis of the prognostic value of AS events and SFs in MPM. The SF\u2013AS regulatory network established in this study and our drug sensitivity analysis using the SF model may provide novel targets for pharmacological studies of MPM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5174","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T06:36:21Z","timestamp":1661754981000},"page":"4895-4906","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Prognostic risk assessment model for alternative splicing events and splicing factors in malignant pleural mesothelioma"],"prefix":"10.1002","volume":"12","author":[{"given":"Yue","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Clinical Medicine Southwest Medical University  Luzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chengda","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology The Third Hospital of Mian 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