{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T12:26:25Z","timestamp":1790511985652,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T00:00:00Z","timestamp":1450915200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23390387"],"award-info":[{"award-number":["23390387"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23791819"],"award-info":[{"award-number":["23791819"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2016,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Exosomes are small membrane vesicles secreted from a variety of cell types. Recent evidence indicates that human cells communicate with each other by exchanging exosomes. Cancer cells closely interact with neighboring stromal cells, and together they cooperatively promote disease via bidirectional communication. Here, we investigated whether exosomes can play roles in intercellular communication between cancer cells and neighboring fibroblasts. Endometrial fibroblasts were isolated from normal endometrial tissues and from endometrial cancer tissues, and cell\u2010to\u2010cell transfer of endometrial cancer cell line Ishikawa\u2010derived exosomes was examined. The isolated fibroblasts were cultured in conditioned media from\n                    <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>\n                    63\u2010\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    \u2010expressing Ishikawa cells, and we found that\n                    <jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\n                    \u2010positive exosomes were transferred from Ishikawa cells to the fibroblasts. Next, we introduced a sh\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    for a luciferase gene into Ishikawa cells. This sh\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    was encapsulated into exosomes, was transferred to the fibroblasts, and then downregulated luciferase expression in the fibroblasts. The mature micro\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s naturally expressed in Ishikawa\u2010derived exosomes were also transported into the endometrial fibroblasts, and they altered the micro\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    expression profiles of the fibroblasts. These results indicated that endometrial cancer cells could transmit small regulatory\n                    <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\n                    s to endometrial fibroblasts via exosomes. Our findings document a previously unknown mode of intercellular communication between cancer cells and related fibroblasts in human endometrium.\n                  <\/jats:p>","DOI":"10.1002\/cam4.545","type":"journal-article","created":{"date-parts":[[2015,12,24]],"date-time":"2015-12-24T00:24:52Z","timestamp":1450916692000},"page":"304-314","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["Exosomal transfer of functional small\n                    <scp>RNA<\/scp>\n                    s mediates cancer\u2010stroma communication in human endometrium"],"prefix":"10.1002","volume":"5","author":[{"given":"Yoshiko","family":"Maida","sequence":"first","affiliation":[{"name":"Department of Obstetrics and Gynecology Kanazawa University School of Medicine  Kanazawa Japan"},{"name":"Department of Molecular Pharmacology Kanazawa University School of Medicine  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Takakura","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Kanazawa University School of Medicine  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takumi","family":"Nishiuchi","sequence":"additional","affiliation":[{"name":"Institute for Gene Research Kanazawa University Advanced Science Research Center  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tanihiro","family":"Yoshimoto","sequence":"additional","affiliation":[{"name":"Department of Molecular Pharmacology Kanazawa University School of Medicine  Kanazawa Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Kyo","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology Shimane University School of Medicine  Izumo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,24]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03096"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1877"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1159\/000218708"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2010.04.032"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2012.03.006"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12307-012-0110-2"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.11.024"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2567"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3335"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1596"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms1180"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1800"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2011.03.043"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604978"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.12.028"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-1489"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.06.010"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2012.08.007"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1722"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)65712-4"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.26"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M110.107821"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12672-015-0223-4"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0068923"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2015.07.004"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1929"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1722"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3166"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.545","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.545","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.545","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.545","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T11:25:38Z","timestamp":1696591538000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.545"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,12,24]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2016,2]]}},"alternative-id":["10.1002\/cam4.545"],"URL":"https:\/\/doi.org\/10.1002\/cam4.545","archive":["Portico"],"relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.726038372.793517738","asserted-by":"object"}]},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,12,24]]},"assertion":[{"value":"2015-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-08-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2015-12-24","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}