{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T11:15:42Z","timestamp":1788952542986,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,11,24]],"date-time":"2022-11-24T00:00:00Z","timestamp":1669248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Backgroud<\/jats:title><jats:p>Pancreatic cancer (PC) is a highly invasive malignancy with extremely poor prognosis. <jats:italic>STK31<\/jats:italic> has been identified as a cancer\u2010testis (CT) gene, but its function in PC has not been elucidated well.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The effect of <jats:italic>STK31<\/jats:italic> on cell proliferation, migration and invasion was investigated by in vitro and in vivo experiments and total RNA sequencing and targeted bisulfite sequencing was applied to explore the potential regulatory mechanisms of <jats:italic>STK31<\/jats:italic> in PC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>By analysis of tissue samples and\u00a0the clinicopathologic features, we found that <jats:italic>STK31<\/jats:italic> was reactivated in PC and associated with poor prognosis. In addition, the vitro and vivo studies indicated that <jats:italic>STK31<\/jats:italic> could promote PC progression by facilitating cell proliferation, migration and invasion, and the indication. Targeted Bisulfite Sequencing showed that <jats:italic>STK31<\/jats:italic> was regulated by methylation. Furthermore, the results of total RNA sequencing suggested that <jats:italic>STK31<\/jats:italic> was closely related to signal transduction, metabolism, and the immune system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrates that <jats:italic>STK31<\/jats:italic>, as a CT gene, can promote the development of PC and is regulated by methylation. <jats:italic>STK31<\/jats:italic> could be considered as a potential therapeutic target for PC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5472","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T07:06:20Z","timestamp":1669359980000},"page":"7273-7282","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Cancer\u2010testis gene <scp><i>STK31<\/i><\/scp> is regulated by methylation and promotes the development of pancreatic cancer"],"prefix":"10.1002","volume":"12","author":[{"given":"Hao","family":"Gao","sequence":"first","affiliation":[{"name":"Pancreas Center the First Affiliated Hospital of Nanjing Medical University  Nanjing China"},{"name":"Pancreas Institution of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baobao","family":"Cai","sequence":"additional","affiliation":[{"name":"Pancreas Center the First Affiliated Hospital of Nanjing Medical University  Nanjing China"},{"name":"Pancreas Institution of Nanjing Medical University  Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zipeng","family":"Lu","sequence":"additional","affiliation":[{"name":"Pancreas Center the First Affiliated Hospital of Nanjing Medical University  Nanjing China"},{"name":"Pancreas Institution of Nanjing Medical University  Nanjing 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