{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T21:50:18Z","timestamp":1790718618363,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In chronic lymphocytic leukemia (CLL), changes in the peripheral blood lymphocyte subsets play an important role in disease progression and infectious complications. The impact of chemoimmunotherapy (CIT) on these changes has not been extensively studied<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used multi\u2010color flow cytometry, to prospectively measure absolute and relative numbers of CD4<jats:sup>+<\/jats:sup> and CD8<jats:sup>+<\/jats:sup> T\u2010cells and their subsets in 45 patients with indolent untreated CLL, 86 patients indicated for first\u2010line treatment, and 34 healthy controls. In 55 patients, we analyzed the impact of CIT<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CLL patients had a significant increase in most cell populations in comparison to controls. Progression of CLL was characterized by significantly elevated counts with the exception of a lower percentage of na\u00efve T\u2010cells. After treatment, the percentage of na\u00efve T\u2010cells further decreased at the expense of effector memory T\u2010cells (TEM). In patients with indolent CLL, higher percentages of na\u00efve CD4<jats:sup>+<\/jats:sup> (<jats:italic>p<\/jats:italic>\u00a0=\u20090.0026) and na\u00efve CD8<jats:sup>+<\/jats:sup> (<jats:italic>p<\/jats:italic>\u00a0=\u20090.023) T\u2010cells were associated with a longer time to first treatment (TTFT). The elevation of CD4<jats:sup>+<\/jats:sup> central memory T\u2010cells (TCM) (<jats:italic>p<\/jats:italic>\u00a0=\u20090.27) and TEM (<jats:italic>p<\/jats:italic>\u00a0=\u20090.003) counts and a higher percentage of CD4<jats:sup>+<\/jats:sup> TEM (<jats:italic>p<\/jats:italic>\u00a0=\u20090.0047), were linked with shorter TTFT. In treated patients, increased regulatory T\u2010cells count was associated with shorter time to next treatment (TTNT) (<jats:italic>p<\/jats:italic>\u00a0=\u20090.042), while higher CD4<jats:sup>+<\/jats:sup> TCM count with shorter TTNT (<jats:italic>p<\/jats:italic>\u00a0=\u20090.035) and shorter overall survival (<jats:italic>p<\/jats:italic>\u00a0=\u20090.041).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that na\u00efve cell depletion and CD4<jats:sup>+<\/jats:sup> TCM and TEM increases are detrimental to CLL patients' prognosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5492","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T10:08:17Z","timestamp":1669630097000},"page":"6956-6970","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["A comprehensive assessment of lymphocyte subsets, their prognostic significance, and changes after first\u2010line therapy administration in patients with chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8050-7594","authenticated-orcid":false,"given":"Pavel","family":"Vod\u00e1rek","sequence":"first","affiliation":[{"name":"4th Department of Internal Medicine \u2013 Hematology Faculty of Medicine University Hospital and Charles University  Hradec Kralove Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6897-8756","authenticated-orcid":false,"given":"Dominika","family":"\u00c9csiov\u00e1","sequence":"additional","affiliation":[{"name":"4th Department of Internal Medicine \u2013 Hematology Faculty of Medicine University Hospital and Charles University  Hradec Kralove Czech 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