{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,2]],"date-time":"2026-09-02T22:24:08Z","timestamp":1788387848669,"version":"build-2803163510"},"reference-count":48,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T00:00:00Z","timestamp":1674518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Noncoding RNAs such as microRNAs (miRNAs) have attracted attention as biological pathway regulators, which differ from chromosomal translocations and gene point mutations. Their involvement in the molecular mechanisms underlying light chain (AL) amyloidosis pathogenesis is yet to be elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>To decipher specific miRNA expression profile in AL\u2010amyloidosis and to examine how miRNAs are involved in AL pathogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The expression profile of miRNAs and mRNA from bone marrow (BM)\u2010derived CD138+ cells were determined using the NanoString nCounter assay and RNA\u2010Seq, respectively. The effect of aberrantly expressed miRNAs on potential molecular targets was analyzed by qRT\u2010PCR, Western blot, Mito\u2010potential assay, and Annexin\u2010PI staining.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Genes which were significantly differentially expressed between AL\u2010amyloidosis and MM, were found to be involved in cell growth and apoptotic mechanisms. Specifically, BCL2L1, MCL1, and BCL2 were upregulated in AL\u2010amyloidosis compared with MM and controls. The levels of miR\u2010181a\u20105p and miR\u20109\u20105p, which regulate the above\u2010mentioned genes, were lower in BM samples from AL\u2010amyloidosis compared with controls, providing a mechanism for BCL2 family gene upregulation. When miR\u20109\u20105p and miR\u2010181a\u20105p were overexpressed in ALMC1 cells, BCL2L1, MCL1, and BCL2 were downregulated and induced apoptosis. Treatment of ALMC\u20101 cells with venetoclax, (BCL\u20102 inhibitor), resulted in the upregulation of those miRNAs, the downregulation of BCL2, MCL1, and BCL2L1 mRNA and protein levels, and subsequent apoptosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings suggest that miR\u20109\u20105p and miR\u2010181a\u20105p act as tumor\u2010suppressors whose downregulation induces anti\u2010apoptotic mechanisms underlying the pathogenesis of AL\u2010amyloidosis. The study highlights the post\u2010transcriptional regulation in AL\u2010amyloidosis and provides pathogenetic evidence for the potential use of BCL\u20102 inhibitors in this disease.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.5621","type":"journal-article","created":{"date-parts":[[2023,1,24]],"date-time":"2023-01-24T23:59:30Z","timestamp":1674604770000},"page":"8199-8210","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>AL<\/scp>\n                    amyloidosis clonal plasma cells are regulated by\n                    <scp>microRNAs<\/scp>\n                    and dependent on anti\u2010apoptotic\n                    <scp>BCL2<\/scp>\n                    family members"],"prefix":"10.1002","volume":"12","author":[{"given":"Hila","family":"Fishov","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Faculty of Natural Sciences Ariel University  Ariel 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