{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,6]],"date-time":"2026-07-06T11:03:13Z","timestamp":1783335793641,"version":"3.54.6"},"reference-count":54,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Myeloid sarcoma (MS) is a rare, extramedullary tumor consisting of myeloid blasts. Little is known about the genetic background of MS and the prognostic value of genetic abnormalities in MS. In particular, the broad variety of gene fusions that occur in MS is marginally covered by traditional testing methods due to lack of fresh tumor specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Here, we analyzed the clinical and genetic features of 61 MS cases. We performed RNA sequencing (RNA\u2010seq) on formalin\u2010fixed paraffin\u2010embedded (FFPE) or fresh samples to analyze fusion genes in 26 cases. In addition, we performed genetic abnormalities\u2010based risk stratification using fusion genes and gene mutations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 305 fusion genes were identified in 22 cases, including the following five recurrent fusion genes: <jats:italic>RUNX1\u2010RUNX1T1<\/jats:italic>, <jats:italic>CBF\u03b2\u2010MYH11<\/jats:italic>, <jats:italic>ETV6\u2010MECOM<\/jats:italic>, <jats:italic>FUS\u2010ERG<\/jats:italic>, and <jats:italic>PICALM\u2010MLLT10<\/jats:italic>. The prognosis in the adverse\u2010risk group was significantly worse than that in the favorable\/intermediate\u2010risk group (median survival: 12\u2009months vs. not reached; <jats:italic>p<\/jats:italic> = 0.0004).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results indicated the efficacy of RNA\u2010seq using FFPE\u2010derived RNA as a clinical routine for detecting fusion genes, which can be used as markers for risk stratification in MS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5654","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T12:37:44Z","timestamp":1678797464000},"page":"9156-9166","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["<scp>RNA<\/scp> sequencing of myeloid sarcoma, shed light on myeloid sarcoma stratification"],"prefix":"10.1002","volume":"12","author":[{"given":"Yunfan","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Hematology, Institute of Hematology West China Hospital of Sichuan University  Chengdu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shu","sequence":"additional","affiliation":[{"name":"Department of Gastrointestinal Surgery, State Key Laboratory of Biotherapy and Cancer Center West China Hospital of Sichuan University  Chengdu People's Republic of China"},{"name":"State Key Laboratory of Biotherapy and Cancer Center West China Hospital of Sichuan University  Chengdu People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan","family":"Tang","sequence":"additional","affiliation":[{"name":"Department of Pathology West China Hospital of Sichuan University  Chengdu People's Republic of 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