{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T10:47:24Z","timestamp":1789469244255,"version":"build-2803163510"},"reference-count":38,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,2,28]],"date-time":"2023-02-28T00:00:00Z","timestamp":1677542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972746"],"award-info":[{"award-number":["81972746"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82000511"],"award-info":[{"award-number":["82000511"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chromosomal heterogeneity leads to the abnormal expression and mutation of tumor\u2010specific genes. Drugs targeting oncogenes have been extensively developed. However, given the random mutation of tumor suppressor genes, the development of its targeted drugs is difficult.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Our early research revealed that artificial circular single\u2010stranded DNA (CSSD) can restore multiple tumor suppressor genes to inhibit tumor malignant progression by adsorbing miRNA. Here, we improved CSSD to a fully closed single\u2010stranded DNA with G quadruplex DNA secondary structure (G4\u2010CSSD), which made G4\u2010CSSD with higher acquisition rate and decreased degradation. The Cancer Genome Atlas (TCGA) and Human Protein Atlas database were used to predict tumour suppressor genes in colon cancer. Cellular and animal experiments were performed to validate the role of G4\u2010CSSD in cancer cell progression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>In colon cancer, we observed the simultaneous low expressions of chloride channel accessory 1 (CLCA1), UDP\u2010GlcNAc:betaGal beta\u20101,3\u2010N\u2010acetylglucosaminyltransferase 6 (B3GNT6) and UDP glucuronosyltransferase family 2 member A3 (UGT2A3), which indicated an favourable prognosis. After repressing miR\u2010590\u20103p with G4\u2010CSSD590, the upregulation of CLCA1, B3GNT6 and UGT2A3 inhibited the proliferation and metastasis of colon cancer cells.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>This study may provide basis for new treatment methods for colon cancer by restoration of tumor suppressor genes.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.5721","type":"journal-article","created":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T02:05:20Z","timestamp":1677636320000},"page":"9774-9787","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["G\u2010quadruplex\u2010enhanced circular single\u2010stranded\n                    <scp>DNA<\/scp>\n                    (\n                    <scp>G4\u2010CSSD<\/scp>\n                    ) adsorption of\n                    <scp>miRNA<\/scp>\n                    to inhibit colon cancer progression"],"prefix":"10.1002","volume":"12","author":[{"given":"Haidong","family":"Wu","sequence":"first","affiliation":[{"name":"Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs and Tianjin Key Laboratory of Molecular Drug Research Tianjin International Joint Academy of Biomedicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weilong","family":"Zhong","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Digestive Diseases, Department of Gastroenterology and Hepatology Tianjin Institute of Digestive Diseases, Tianjin Medical University General Hospital  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronghua","family":"Zhang","sequence":"additional","affiliation":[{"name":"Hebei Provincial Key Laboratory of Medical\u2010Industrial Integration Precision Medicine, School of Clinical Medicine North China University of Science and Technology  Tangshan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuping","family":"Ding","sequence":"additional","affiliation":[{"name":"Gastroenterology Department of Medical Center of CAPF  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunhua","family":"Qu","sequence":"additional","affiliation":[{"name":"Department of outpatient and emergency Shanghai Pudong Hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keguan","family":"Lai","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs and Tianjin Key Laboratory of Molecular Drug Research Tianjin International Joint Academy of Biomedicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng","family":"Pang","sequence":"additional","affiliation":[{"name":"Tianjin Key Laboratory of Early Druggability Evaluation of Innovative Drugs and Tianjin Key Laboratory of Molecular Drug Research Tianjin International Joint Academy of Biomedicine  Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan","family":"Yin","sequence":"additional","affiliation":[{"name":"OBiO Technology (Shanghai) Co., Ltd.  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