{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T08:28:25Z","timestamp":1765268905973,"version":"3.40.5"},"reference-count":28,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["2021R1A2C4086635"],"award-info":[{"award-number":["2021R1A2C4086635"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Urine cytology is the most widely used noninvasive screening tool for urothelial carcinoma diagnosis and surveillance. Although highly specific, urine cytology exhibits suboptimal sensitivity. This study aimed to determine whether hTERT immunocytochemistry (ICC) could be applicable as an ancillary test in routine cytology practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 561 urinary tract samples were initially screened in this study. All of them were prepared using SurePath liquid\u2010based cytology (LBC), while additional LBC slides were made and subsequently used for hTERT (SCD\u2010A7) ICC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From the 561 samples screened, 337 were finally analyzed, all having an adequate cellularity and available follow\u2010up histology. The hTERT ICC\u2010positive rate was 95.9% (<jats:italic>n<\/jats:italic>\u00a0=\u00a0208\/217), 96% (<jats:italic>n<\/jats:italic>\u00a0=\u00a024\/25), and 100% (<jats:italic>n<\/jats:italic>\u00a0=\u00a04\/4) in cytology samples with high\u2010grade urothelial carcinoma, carcinoma in situ, and low\u2010grade urothelial carcinoma subsequent histology. Among the 64 atypical cytology cases histologically confirmed as urothelial carcinomas, 92.2% (<jats:italic>n<\/jats:italic>\u00a0=\u00a059\/64) were immunoreactive to hTERT, whereas the two histologically benign cases were ICC\u2010negative. 87\/90 (96.7%) of the cytology cases confirmed to be benign in follow\u2010up were hTERT\u2010negative. The overall sensitivity and specificity of hTERT ICC were 96.3% and 98.8%, respectively (AUROC\u00a0=\u00a00.963; 95% CI\u00a0=\u00a00.960\u20130.967).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The hTERT ICC test exhibited consistent and intense staining in malignant urothelial cells, suggesting its value as an ancillary test in liquid\u2010based urine cytology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5767","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T09:50:12Z","timestamp":1678787412000},"page":"10363-10370","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Clinical application of the anti\u2010human telomerase reverse transcriptase (h<scp>TERT<\/scp>) antibody (<scp>SCD\u2010A7<\/scp>) immunocytochemistry in liquid\u2010based urine cytology: A\u00a0prospective, single institute study"],"prefix":"10.1002","volume":"12","author":[{"given":"Ji Hye","family":"Moon","sequence":"first","affiliation":[{"name":"Department of Pathology, Kangbuk Samsung Hospital Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilias P.","family":"Nikas","sequence":"additional","affiliation":[{"name":"School of Medicine European University Cyprus  Nicosia Cyprus"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyung Chul","family":"Moon","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Pathology Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bohyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Pathology, Konkuk University Medical Center Konkuk University School of Medicine  Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2508-9796","authenticated-orcid":false,"given":"Han Suk","family":"Ryu","sequence":"additional","affiliation":[{"name":"Department of Pathology Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Pathology Seoul National University College of Medicine  Seoul Republic of Korea"},{"name":"Center for Medical Innovation, Biomedical Research Institute Seoul National University Hospital  Seoul Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"issue":"2","key":"e_1_2_10_2_1","first-page":"163","article-title":"Accuracy of urinary cytology in the diagnosis of primary and recurrent bladder cancer","volume":"37","author":"Wiener HG","year":"1993","journal-title":"Acta 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