{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T02:59:42Z","timestamp":1772765982374,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T00:00:00Z","timestamp":1682035200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["7182032"],"award-info":[{"award-number":["7182032"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81672819"],"award-info":[{"award-number":["81672819"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81773538"],"award-info":[{"award-number":["81773538"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Effective noninvasive biomarkers of gastric cancer (GC) are critical for early detection and improvement of prognosis. We performed genome\u2010wide long non\u2010coding RNA (lncRNA) microarray analysis to identify and validate novel GC biomarkers depending on a high\u2010risk population cohort.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>LncRNA profiles were described using the Human LncRNA Microarray between GC and control plasma samples. The differential candidate lncRNAs were validated in two stages by quantitative reverse transcription polymerase chain reaction (qRT\u2010PCR). We further evaluated the joint effect between the GC\u2010associated lncRNA and <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>) infection on the risk of cardia and non\u2010cardia GC, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Different lncRNA expression profiles were identified between GC and control plasma with a total of 1206 differential lncRNAs including 470 upregulated and 736 downregulated in GC compared with the control group. The eight significantly upregulated lncRNAs (<jats:italic>RP11\u2010521D12.1<\/jats:italic>, <jats:italic>AC011995.3<\/jats:italic>, <jats:italic>RP11\u20105P4.3<\/jats:italic>, <jats:italic>RP11\u2010244\u2009K5.6<\/jats:italic>, <jats:italic>RP11\u2010422\u2009J15.1<\/jats:italic>, <jats:italic>CTD\u20102306\u2009M5.1<\/jats:italic>, <jats:italic>CTC\u2010428G20.2<\/jats:italic>, and <jats:italic>AC009133.20<\/jats:italic>) in GC cases both in the present study and a similar microarray screening study by our collaborative team were selected for a two\u2010stage validation. After the large sample size validation, the subjects with higher expression of <jats:italic>RP11\u2010244\u2009K5.6<\/jats:italic> showed a significantly increased risk of GC with an adjusted odds ratio (OR) as 2.68 and 95% confidence interval (CI) as 1.15\u20136.24. Joint effects between <jats:italic>RP11\u2010244\u2009K5.6<\/jats:italic> expression and <jats:italic>H. pylori<\/jats:italic> infection on the risk of GC were evaluated with no statistical significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study found different lncRNA expression profiles between GC and control plasma and preliminarily identified <jats:italic>RP11\u2010244\u2009K5.6<\/jats:italic> as a potential noninvasive biomarker for GC screening.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5905","type":"journal-article","created":{"date-parts":[[2023,4,21]],"date-time":"2023-04-21T13:10:02Z","timestamp":1682082602000},"page":"12728-12738","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gastric cancer\u2010associated long non\u2010coding RNA profiling and noninvasive biomarker screening based on a high\u2010risk population cohort"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4077-1868","authenticated-orcid":false,"given":"Xiaoying","family":"Guo","sequence":"first","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; 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Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wenqing","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weicheng","family":"You","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; Institute  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoquan","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of General Surgery, The Affiliated Huai'an No.1 People's Hospital Nanjing Medical University  Huai'an 223300 China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1159-1884","authenticated-orcid":false,"given":"Wanqing","family":"Chen","sequence":"additional","affiliation":[{"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hongmei","family":"Zeng","sequence":"additional","affiliation":[{"name":"National Cancer Center\/National Clinical Research Center for Cancer\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College  Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kaifeng","family":"Pan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education\/Beijing), Department of Cancer Epidemiology, Peking University School of Oncology Peking University Cancer Hospital &amp; 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