{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T15:08:02Z","timestamp":1777388882707,"version":"3.51.4"},"reference-count":46,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T00:00:00Z","timestamp":1682380800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Abnormal activation of Wnt\/\u03b2\u2010catenin signaling is associated with various aspects of cancer development. This study explored the roles of novel target genes of the Wnt\/\u03b2\u2010catenin signaling pathway in cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Using the haploid chronic myelogenous leukemia cell line HAP1, RNA sequencing (RNA\u2010seq) was performed to identify genes whose expression was increased by <jats:italic>APC<\/jats:italic> disruption and reversed by \u03b2\u2010catenin knockdown (KD). The regulatory mechanism and function of one of the candidate genes was investigated in colorectal cancer (CRC) cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In total, 64 candidate genes whose expression was regulated by Wnt\/\u03b2\u2010catenin signaling were identified. Of these candidate genes, the expression levels of six were reduced by \u03b2\u2010catenin KD in HCT116 CRC cells in our previous microarray. One of these genes was <jats:italic>Visinin\u2010like 1<\/jats:italic> (<jats:italic>VSNL1<\/jats:italic>), which belongs to the neuronal calcium\u2010sensor gene family. The expression of <jats:italic>VSNL1<\/jats:italic> was regulated by the \u03b2\u2010catenin\/TCF7L2 complex via two TCF7L2\u2010binding elements in intron 1. VSNL1 KD\u2010induced apoptosis in VSNL1\u2010positive CRC cells. Additionally, forced expression of wild\u2010type VSNL1, but not a myristoylation, Ca<jats:sup>2+<\/jats:sup>\u2010binding, or dimerization\u2010defective mutant, suppressed the apoptosis induced by camptothecin and doxorubicin in VSNL1\u2010negative CRC cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that <jats:italic>VSNL1<\/jats:italic>, a novel target gene of the Wnt\/\u03b2\u2010catenin signaling pathway, is associated with apoptosis resistance in CRC cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.5970","type":"journal-article","created":{"date-parts":[[2023,4,25]],"date-time":"2023-04-25T11:59:16Z","timestamp":1682423956000},"page":"13426-13437","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Visinin\u2010like 1<\/i>, a novel target gene of the Wnt\/\u03b2\u2010catenin signaling pathway, is involved in apoptosis resistance in colorectal 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