{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:33:51Z","timestamp":1786977231986,"version":"build-2736575974"},"reference-count":57,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T00:00:00Z","timestamp":1683158400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      B\u2010cell chronic lymphocytic leukemia (B\u2010CLL) is characterized by the expansion of CD5\n                      <jats:sup>+<\/jats:sup>\n                      malignant B lymphocytes. Recent discoveries have shown that double\u2010negative T (DNT) cells, double\u2010positive T (DPT) cells, and natural killer T (NKT)\u2010cells may be involved in tumor surveillance.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>A detailed immunophenotypic analysis of the peripheral blood T\u2010cell compartment of 50 patients with B\u2010CLL (classified in three prognostic groups) and 38 healthy donors (as controls) matched for age was performed. The samples were analyzed by flow cytometry using a stain\u2010lyse\u2010no wash technique and a comprehensive six\u2010color antibody panels.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Our data confirmed a reduction in percentage values and an increase in absolute values of T lymphocytes in patients with B\u2010CLL, as already reported. In particular, DNT, DPT, and NKT\u2010like percentages were significantly lower than in the controls, except for NKT\u2010like in the low\u2010risk prognostic group. Moreover, a significant rise in the absolute counts of DNT cells in each prognostic group and in the low\u2010risk prognostic group of NKT\u2010like cells was found. A significant correlation of the absolute values of NKT\u2010like cells in the intermediate\u2010risk prognostic group versus B cells was observed. Furthermore, we analyzed whether the increase in T cells was related to the subpopulations of interest. Only DNT cells were positively correlated with the increase in CD3\n                      <jats:sup>+<\/jats:sup>\n                      T lymphocytes, regardless of the stage of the disease, supporting the hypothesis that this T\u2010cell subset plays a key role in the immune T response in B\u2010CLL.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These early results supported that DNT, DPT, and NKT\u2010like subsets may be related to disease progression and should encourage further studies aimed at identifying the potential immune surveillance role of these minority T subpopulations.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.6015","type":"journal-article","created":{"date-parts":[[2023,5,4]],"date-time":"2023-05-04T09:52:00Z","timestamp":1683193920000},"page":"13241-13255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Preliminary analysis of double\u2010negative T, double\u2010positive T, and natural killer T\u2010like cells in B\u2010cell chronic lymphocytic leukemia"],"prefix":"10.1002","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6382-7318","authenticated-orcid":false,"given":"Luciana","family":"Valvano","sequence":"first","affiliation":[{"name":"Laboratory of Clinical Research and Advanced Diagnostics Centro di Riferimento Oncologico della Basilicata (IRCCS\u2010CROB)  Rionero in Vulture Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Filomena","family":"Nozza","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Research and Advanced Diagnostics Centro di Riferimento Oncologico della Basilicata (IRCCS\u2010CROB)  Rionero in Vulture Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"D'Arena","sequence":"additional","affiliation":[{"name":"Immunohematology and transfusional medicine \"S. 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