{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T23:29:26Z","timestamp":1779924566004,"version":"3.53.1"},"reference-count":37,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T00:00:00Z","timestamp":1683676800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Propofol is a drug with potential anticancer effect. This study aimed to explore the effect of propofol on chemosensitivity of cervical cancer cells to paclitaxel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>HeLa and CaSki cells were selected for drug experiments. Cell viability was evaluated via CCK\u20108 assay, and the combination index (CI) was calculated by CompuSyn software. A clinically relevant concentration and IC30 of propofol were selected in combination with 5\u2009nM paclitaxel. BrdU incorporation, transwell, and flow cytometry assays were utilized to evaluate cell proliferation, migration, invasion, and apoptosis. The expression of \u03b2\u2010tubulin, stathmin 1, and GAPDH proteins was evaluated by Western blot. The stathmin 1 cDNA plasmid was used to establish stathmin 1\u2010overexpressing CaSki cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>At clinically relevant concentrations (0\u201380\u2009\u03bcM), propofol did not affect cancer cell viability, but high concentrations (100\u2013800\u2009\u03bcM) reduced cell viability. The CI values of propofol with IC30 (200\u2009\u03bcM in HeLa; 400\u2009\u03bcM in CaSki) combined with 5\u2009nM paclitaxel were\u2009&lt;1. The effect of propofol with IC30 combined with paclitaxel on cell proliferation, migration, invasion, and apoptosis were stronger than individual effect, while 30\u2009\u03bcM propofol had no effect. The Western blot results showed 30\u2009\u03bcM propofol did not affect \u03b2\u2010tubulin and stathmin 1 expression in cells, although paclitaxel upregulated \u03b2\u2010tubulin expression while downregulating stathmin 1 expression. Compared with paclitaxel alone, cotreatment with propofol at its IC30 and paclitaxel decreased stathmin 1 expression but had no effect on \u03b2\u2010tubulin expression. High stathmin 1 expression weakened the effect of paclitaxel on cell viability and apoptosis, while propofol partially reversed these effect.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Propofol at clinically relevant concentrations had no effect on the malignant biological behaviors of cervical cancer cells, while propofol at high concentrations decreased.Propofol with IC30 and paclitaxel had synergetic effect on cancer cells through a reduction in stathmin 1 expression.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.6064","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T04:23:58Z","timestamp":1683779038000},"page":"14403-14412","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["The effect of propofol on chemosensitivity of paclitaxel in cervical cancer cells"],"prefix":"10.1002","volume":"12","author":[{"given":"Yanshan","family":"Jin","sequence":"first","affiliation":[{"name":"Center for Uterine Cancer Diagnosis &amp; Therapy Research of Zhejiang Province, Department of Obstetrics and Gynecology The Second Affiliated Hospital of Wenzhou Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9789-233X","authenticated-orcid":false,"given":"Shangdan","family":"Xie","sequence":"additional","affiliation":[{"name":"Center for Uterine Cancer Diagnosis &amp; Therapy Research of Zhejiang Province, Department of Obstetrics and Gynecology The Second Affiliated Hospital of Wenzhou Medical University  Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Sheng","sequence":"additional","affiliation":[{"name":"Center for Uterine Cancer Diagnosis &amp; Therapy Research of Zhejiang Province, Department of Obstetrics and Gynecology The 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