{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T15:24:30Z","timestamp":1770909870123,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T00:00:00Z","timestamp":1455580800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100008731","name":"Nakatomi Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2016,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cetuximab is remarkable for the relatively high rate and severity of hypersensitivity reactions (<jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>) being reported in the literature. Screening for cetuximab\u2010specific IgE in serum via immunoassay has been found to be useful in preventing <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>; however, these tests are known to have a low positive predictive rate. In an attempt to remedy this, we evaluated the interaction between cetuximab and IgE on basophils for predicting severe cetuximab\u2010induced <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>. Twelve head and neck cancer patients were enrolled in this single\u2010institution study: four with a history of cetuximab\u2010induced <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content> and eight with no such history. Cetuximab\u2010specific and galactose\u2010<jats:italic>\u03b1<\/jats:italic>\u20101,3\u2010galactose (<jats:italic>\u03b1<\/jats:italic>\u2010gal) specific IgEs in serum were measured in vitro using an enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>). IgE\u2010cetuximab binding on basophils was also analyzed to evaluate the decrease in cetuximab molecules on basophils after dissociation of IgE from Fc\u03b5<jats:styled-content style=\"fixed-case\">RI<\/jats:styled-content>. The positive predictive value associated with the presence of cetuximab\u2010 or <jats:italic>\u03b1<\/jats:italic>\u2010gal\u2010specific IgE in serum was found to be only 0.67, whereas the negative predictive value was 1.00. On the other hand, in all four patients who developed <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>, the cetuximab molecules on basophils were decreased significantly due to the dissociation of IgE from basophils (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05). However, this was not the case in patients who did not develop <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>. In conclusion, our results strongly imply that the IgE\u2010cetuximab interaction on basophils may be key to developing improved methods for predicting severe cetuximab\u2010induced <jats:styled-content style=\"fixed-case\">HR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1002\/cam4.658","type":"journal-article","created":{"date-parts":[[2016,2,16]],"date-time":"2016-02-16T09:35:19Z","timestamp":1455615319000},"page":"1004-1012","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["A novel approach to predict cetuximab\u2010induced hypersensitivity reaction: detection of drug\u2010specific IgE on basophils"],"prefix":"10.1002","volume":"5","author":[{"given":"Takuya","family":"Iwamoto","sequence":"first","affiliation":[{"name":"Department of Pharmacy Mie University Hospital  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akiharu","family":"Okamoto","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Mie University Hospital  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hajime","family":"Ishinaga","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Mie University Graduate School of Medicine  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kasumi","family":"Shimizu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Mie University Graduate School of Medicine  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto A.","family":"Gayle","sequence":"additional","affiliation":[{"name":"Center for Medical and Nursing Education Mie University Graduate School of Medicine  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoya","family":"Arai","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Mie University Graduate School of Medicine  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiko","family":"Takeuchi","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology\u2010Head and Neck Surgery Mie University Graduate School of Medicine  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Okuda","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Mie University Hospital  Tsu Mie 514\u20108507 Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,2,16]]},"reference":[{"key":"e_1_2_7_2_1","unstructured":"Company B\u2010MS.ERBITUX\u00ae (cetuximab) injection.2013."},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa033025"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa053422"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0802656"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(09)60569-9"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaip.2014.12.006"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2007.11.7812"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/1078155213510542"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.4137\/CMO.S13897"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.333"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.111.S3.1601"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa074943"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.iac.2015.01.009"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.4161\/mabs.3.4.16293"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-7961-3-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2222.2010.03572.x"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cr.7290301"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-7961-6-12"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/all.12344"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1159\/000126061"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.0906-6705.2006.00404.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.2332\/allergolint.O-07-506"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.12538"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.65.1082"},{"key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1159\/000161586","article-title":"Asymptomatic sensitization to hymenoptera venom is related to total immunoglobulin E levels","volume":"148","author":"Sturm G. J.","year":"2009","journal-title":"Int Arch Allergy Immunol."},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ajh.23329"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2014.01.024"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.b12-00150"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0108619"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200968"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.24945"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.658","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.658","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.658","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.658","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T18:02:35Z","timestamp":1696615355000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.658"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,2,16]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1002\/cam4.658"],"URL":"https:\/\/doi.org\/10.1002\/cam4.658","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,2,16]]},"assertion":[{"value":"2015-12-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-02-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}