{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:44:06Z","timestamp":1791409446317,"version":"4.3.3"},"reference-count":31,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,3,6]],"date-time":"2016-03-06T00:00:00Z","timestamp":1457222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001665","name":"Agence Nationale de la Recherche","doi-asserted-by":"publisher","award":["ANR\u201011\u2010LABX\u20100028\u201001"],"award-info":[{"award-number":["ANR\u201011\u2010LABX\u20100028\u201001"]}],"id":[{"id":"10.13039\/501100001665","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004097","name":"Fondation ARC pour la Recherche sur le Cancer","doi-asserted-by":"publisher","award":["ARC SL220110603478"],"award-info":[{"award-number":["ARC SL220110603478"]}],"id":[{"id":"10.13039\/501100004097","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006331","name":"Cancerop\u00f4le PACA","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006331","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2016,6]]},"abstract":"Abstract<\/jats:title>Circulating tumors cells (CTC<\/jats:styled-content>s) can be detected in the blood of metastatic melanoma patients (MMP<\/jats:styled-content>s) both as isolated circulating tumor cells (iCTC<\/jats:styled-content>s) and circulating tumor microemboli (CTM<\/jats:styled-content>s), but their clinical significance remains unknown. The aim of this work was to evaluate the prognostic impact in metastatic cutaneous melanoma of CTM<\/jats:styled-content>s and iCTC<\/jats:styled-content>s identified by a cytomorphological approach using the isolation by size of tumor cell (ISET<\/jats:styled-content>) method. We characterized the phenotype of CTC<\/jats:styled-content>s using anti\u2010PS<\/jats:styled-content>100, anti\u2010SOX<\/jats:styled-content>10, anti\u2010CD<\/jats:styled-content>10, and anti\u2010TRF<\/jats:styled-content>2 antibodies. 128 MMP<\/jats:styled-content>s and 37 control healthy individuals with benign nevi were included in this study. Results were compared to the follow\u2010up of patients. 109\/128 (85%) MMP<\/jats:styled-content>s showed CTC<\/jats:styled-content>s, 44\/128 (34%) with 2 to 6 CTM<\/jats:styled-content>s and 65\/128 (51%) with 4 to 9 iCTC<\/jats:styled-content>s. PS<\/jats:styled-content>100 expression was homogeneous in iCTC<\/jats:styled-content>s and heterogeneous in CTM<\/jats:styled-content>s. SOX<\/jats:styled-content>10, CD<\/jats:styled-content>10, and TRF<\/jats:styled-content>2 were mainly expressed in CTM<\/jats:styled-content>s. None of the control subjects demonstrated circulating malignant tumor cells. Overall survival was significantly decreased in patients with CTM<\/jats:styled-content>s, independently of the therapeutic strategies. In conclusion, the presence of CTM<\/jats:styled-content>s is an independent predictor of shorter survival from the time of diagnosis of MMPs.<\/jats:p>","DOI":"10.1002\/cam4.661","type":"journal-article","created":{"date-parts":[[2016,3,6]],"date-time":"2016-03-06T13:06:37Z","timestamp":1457269597000},"page":"1022-1030","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["High expression of TRF<\/scp>2, SOX<\/scp>10, and CD<\/scp>10 in circulating tumor microemboli detected in metastatic melanoma patients. A potential impact for the assessment of disease aggressiveness"],"prefix":"10.1002","volume":"5","author":[{"given":"Elodie","family":"Long","sequence":"first","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"},{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marius","family":"Ilie","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"},{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"},{"name":"Human Biobank Pasteur Hospital BB\u20100033\u201000025 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Coraline","family":"Bence","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Butori","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Selva","sequence":"additional","affiliation":[{"name":"Human Biobank Pasteur Hospital BB\u20100033\u201000025 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Salom\u00e9","family":"Lalv\u00e9e","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christelle","family":"Bonnetaud","sequence":"additional","affiliation":[{"name":"Human Biobank Pasteur Hospital BB\u20100033\u201000025 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Poissonnet","sequence":"additional","affiliation":[{"name":"Department of Surgery Comprehensive Cancer Center Antoine Lacassagne Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Philippe","family":"Lacour","sequence":"additional","affiliation":[{"name":"Department of Dermatology Archet II Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Bahadoran","sequence":"additional","affiliation":[{"name":"Department of Dermatology Archet II Hospital Nice France"},{"name":"INSERM U1065 Team 1 University of Nice Sophia Antipolis Equipe Labellis\u00e9e Ligue 2013 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Brest","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Gilson","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"},{"name":"Unit of Genetics Archet Hospital Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Ballotti","sequence":"additional","affiliation":[{"name":"INSERM U1065 Team 1 University of Nice Sophia Antipolis Equipe Labellis\u00e9e Ligue 2013 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Hofman","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"},{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"},{"name":"Human Biobank Pasteur Hospital BB\u20100033\u201000025 Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Hofman","sequence":"additional","affiliation":[{"name":"Institute for Research on Cancer and Aging in Nice (IRCAN) INSERM U1081\/CNRS UMR7284 University of Nice Sophia Antipolis Antoine Lacassagne Cancer Center Nice France"},{"name":"Laboratory of Clinical and Experimental Pathology Pasteur Hospital Nice France"},{"name":"Human Biobank Pasteur Hospital BB\u20100033\u201000025 Nice France"},{"name":"Cancer Research Association (ARC) Labelled Team Villejuif France"},{"name":"\u201cOncoAge\u201d Hospital\u2010University Federation CHU Nice France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,3,6]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2012.194258"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2012.00069"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.02.0958"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gde.2009.12.002"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010021"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0041052"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.34"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2013.253"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1999.17.1.304"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0b013e32833906e3"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdu207"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2010.141"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.485"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1097\/CMR.0000000000000025"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.7785\/tcrt.2012.500328"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2012.468"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2015.127"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2013.02.007"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.128"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/pcmr.12028"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2010.10.019"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2012.11.003"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2006.03.010"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-0445"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mds137"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.128"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.1047"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb2774"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2014.55.3487"},{"key":"e_1_2_7_31_1","first-page":"000","article-title":"Neutrophil extracellular traps sequester circulating tumor cells and promote metastasis","volume":"0000","author":"Cools\u2010Lartigue J.","year":"2013","journal-title":"J. Clin. Invest."},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/1742-4682-11-17"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fcam4.661","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.661","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T18:02:14Z","timestamp":1696615334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,3,6]]},"references-count":31,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2016,6]]}},"alternative-id":["10.1002\/cam4.661"],"URL":"https:\/\/doi.org\/10.1002\/cam4.661","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,3,6]]},"assertion":[{"value":"2015-07-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-01-13","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-03-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}