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A doxorubicin\u2010resistant HCT\u20108\/R clone was selected from sensitive parental cells and characterized analyzing several parameters (cell cycle phase distribution, apoptotic activity, expression, distribution and functionality of the P\u2010gp efflux pump, the response to other chemotherapy agents, its ultrastructural features, invasiveness, and transcriptomic profile). HCT\u20108\/R cells showed a peculiar S phase distribution, characterized by a single pulse of proliferation, resistance to drug\u2010mediated apoptosis, increased expression and functionality of P\u2010gp and overexpression of stem cell markers (CD44 and aldehyde dehydrogenase 1A2). At the ultrastructural level, HCT\u20108\/R presented a greater cell volume and several intracytoplasmic vesicles respect to HCT\u20108. Moreover, the resistant clone was characterized by cross resistance to other cytotoxic drugs and a greater capacity for migration and invasion, compared to parental cells. Our data reinforce the concept that the MDR phenotype in HCT\u20108\/R cells is multifactorial and involves multiple mechanisms, representing an interesting tool to understand the biological basis of MDR and to test strategies that overcome resistance to chemotherapy.<\/jats:p>","DOI":"10.1002\/cam4.694","type":"journal-article","created":{"date-parts":[[2016,3,26]],"date-time":"2016-03-26T11:35:06Z","timestamp":1458992106000},"page":"1279-1291","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Development and characterization of an in vitro model of colorectal adenocarcinoma with MDR phenotype"],"prefix":"10.1002","volume":"5","author":[{"given":"Lorenzo","family":"Cinci","sequence":"first","affiliation":[{"name":"Departments of Neuroscience, Psychology, Drug Research and Child Health\u2010NEUROFARBA, Section of Pharmacology and Toxicology University of Florence  Viale G. 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Pieraccini 6 Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilaria","family":"Carboni","sequence":"additional","affiliation":[{"name":"Diagnostic Genetics Unit Azienda Ospedaliero Universitaria \u201cCareggi\u201d  Largo Brambilla 5 Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Paccosi","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Clinical Pharmacology and Oncology Section University of Florence  Viale G. Pieraccini 6 Florence Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Astrid","family":"Parenti","sequence":"additional","affiliation":[{"name":"Department of Health Sciences, Clinical Pharmacology and Oncology Section University of Florence  Viale G. 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