{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T18:56:48Z","timestamp":1780081008224,"version":"3.54.0"},"reference-count":34,"publisher":"Wiley","issue":"18","license":[{"start":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T00:00:00Z","timestamp":1727136000000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100009576","name":"Nantong Municipal Commission of Health and Family Planning","doi-asserted-by":"publisher","award":["MS2022069"],"award-info":[{"award-number":["MS2022069"]}],"id":[{"id":"10.13039\/501100009576","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100009576","name":"Nantong Municipal Commission of Health and Family Planning","doi-asserted-by":"publisher","award":["MSZ2023036"],"award-info":[{"award-number":["MSZ2023036"]}],"id":[{"id":"10.13039\/501100009576","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Previous studies have demonstrated that the glycolytic enzyme phosphoglycerate kinase 1 (PGK1) can promote tumor development. This study sought to investigate the specific role of PGK1 in bladder cancer (BLCA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Public databases and immunohistochemistry assays were utilized to analyze the expression of PGK1 in BLCA and its prognostic significance. Cell proliferation was assessed through CCK\u20108 and colony formation assays, while the level of metastasis was evaluated using transwell migration experiments. Additionally, IC<jats:sub>50<\/jats:sub> experiments were conducted to assess the impact of PGK1 on cisplatin sensitivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mRNA and protein expression levels of PGK1 were significantly upregulated in BLCA. Cox proportional hazards model analysis revealed that PGK1 and T stage were independent prognostic factors for BLCA patients. Both CCK\u20108 and colony assays demonstrated that PGK1 promotes proliferation. Furthermore, a positive correlation was observed between PGK1 and Ki67, a proliferation index. Transwell migration assays confirmed the ability of PGK1 to enhance metastasis. Finally, PGK1 increased the IC<jats:sub>50<\/jats:sub> associated with cisplatin treatment in BLCA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Collectively, these findings suggest that PGK1 may hold clinical value in predicting BLCA prognosis and improving the outcomes of this patient population.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70242","type":"journal-article","created":{"date-parts":[[2024,9,24]],"date-time":"2024-09-24T13:03:28Z","timestamp":1727183008000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>PGK1<\/scp> can affect the prognosis and development of bladder cancer"],"prefix":"10.1002","volume":"13","author":[{"given":"Mingde","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Urology Affiliated Tumor Hospital of Nantong University and Nantong Tumor Hospital  Nantong People's Republic of 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