{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:06:22Z","timestamp":1753862782513,"version":"3.41.2"},"reference-count":43,"publisher":"Wiley","issue":"19","license":[{"start":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T00:00:00Z","timestamp":1728432000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Abnormal glycosylation is associated with tumors. The clinical value of serum glycans in assessing progression of hepatocellular carcinoma (HCC) patients remains a challenge.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A study dynamically comparing levels of fifteen lectin\u2010specific glycans between preoperative and postoperative serum of 65 HCC patients was conducted via lectin biochip technology. Multivariable logistic regression analysis was used to address associations between serum glycan levels and clinicopathological characteristics. Kaplan\u2013Meier analysis was used to evaluate the impacts of serum glycan levels on overall survival (OS) and progression\u2010free survival (PFS) of the HCC patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HCC patients presented significantly higher levels of the lectin\u2010specific glycans in preoperative serum than disease\u2010free individuals (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001 \u2212 <jats:italic>p<\/jats:italic>\u2009=\u20090.029), except ConA. The glycans in preoperative sera were significantly related to tumor size, pTNM, metastasis, BCLC stage, portal hypertension (PHT), and platelet count (PLT), respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). Multivariate logistic analyses indicated that tumor size and pTNM independently impact on glycan\u2010specific lectins either LTL, UEA\u2010I, VVL, NPL, WGA, PNA, MAL\u2010I, SNA, or PHA\u2010L (<jats:italic>p<\/jats:italic>\u2009=\u20090.003 \u2212 <jats:italic>p<\/jats:italic>\u2009=\u20090.044); BCLC stage and PLT were independent factors influencing the serum glycans recognizable DSA (<jats:italic>p<\/jats:italic>\u2009=\u20090.024) and SNA (<jats:italic>p<\/jats:italic>\u2009=\u20090.050), respectively. Surgical excision of tumor mass significantly reduced glycan levels in sera. Tumor differentiation, albumin, and ABO type significantly revealed independent influence on glycan\u2010specific lectins, such as RCA\u2010I (<jats:italic>p<\/jats:italic>\u2009=\u20090.024), VVL (<jats:italic>p<\/jats:italic>\u2009=\u20090.024), and Con A (<jats:italic>p<\/jats:italic>\u2009=\u20090.026) in the postoperative serum. HCC patients with high levels of VVL\u2010binding glycans significantly benefited from a longer OS time (<jats:italic>p<\/jats:italic>\u2009=\u20090.016, HR: 0.460, 95% CI: 0.237\u20130.892) and a better PFS time (<jats:italic>p<\/jats:italic>\u2009=\u20090.004; HR: 0.435, 95% CI: 0.237\u20130.799), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Serum glycans could reflect surgical outcomes in at\u2010risk patients and become valuable biomarkers in evaluating the progression of HCC patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70285","type":"journal-article","created":{"date-parts":[[2024,10,9]],"date-time":"2024-10-09T12:44:21Z","timestamp":1728477861000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Preoperative Serum Glycan Levels Reflect Progression of Patients With Hepatocellular Carcinoma"],"prefix":"10.1002","volume":"13","author":[{"given":"Sheng\u2010Sheng","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital Anhui Medical University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lei","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery The First Affiliated Hospital, Anhui Medical University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qing\u2010Qing","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Biliary\u2010Pancreas Surgery The First Affiliated Hospital, Anhui Medical University  Hefei China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital Anhui Medical University  Hefei China"},{"name":"Department of Pathology Anhui 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