{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,15]],"date-time":"2026-01-15T00:16:37Z","timestamp":1768436197192,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T00:00:00Z","timestamp":1731024000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","award":["MG01\/21"],"award-info":[{"award-number":["MG01\/21"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Pancreatic cancer (PC) is one of the most aggressive types of cancer. Despite advances in molecular diagnostics, PC diagnosis relies on imaging technologies and morphological assessment of fine needle aspirates (FNAs). MicroRNA (miRNA) involvement in PC pathogenesis and potential diagnostics application have been suggested, albeit current supporting evidence is lacking. Here, we investigated the association of selected miRNAs with PC incidence and clinical characteristics.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Fold expression of miR\u2010216a\u20103p, \u2010217\u20105p, \u2010221\u20103p, \u2010222\u20103p, and miR\u2010196a\u20105p was assessed in 73 PC FNA cell\u2010block sections and 6 healthy pancreas tissues using Taqman advanced miRNA assays. Potential miRNA targets were ascertained using immunocytochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      miR\u2010196a\u20105p was upregulated in PC compared to healthy pancreatic tissue (\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2009=\u2009\u22120.05, 95% CI: \u22120.065 \u2013 (\u22120.035);\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). miR\u2010221\u20103p and miR\u2010222\u20103p fold expression were strongly correlated (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.897,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), whereas miR\u2010196a\u20105p fold expression correlated with that of miR\u2010221\u20103p (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.688,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) and miR\u2010222\u20103p (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.489,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001). Moreover, miR\u2010196a\u20105p fold expression positively correlated with tumor stage (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u20090.32,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.034). miR\u2010217\u20105p fold expression inversely correlated with KRAS expression (\n                      <jats:italic>r<\/jats:italic>\n                      \u2009=\u2009\u20100.69,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.0027).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our study shows miR\u2010196a\u20105p has reasonable specificity to PC and thus may have diagnostic and prognostic potential in PC as proposed in the literature. Moreover, KRAS and NFKBIA may be potential targets for miR\u2010217\u20105p and miR\u2010196a\u20105p, respectively. Thus, these miRNAs may be involved in tumor progression and may have valuable applications in novel therapeutics or treatment monitoring.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.70400","type":"journal-article","created":{"date-parts":[[2024,11,8]],"date-time":"2024-11-08T05:27:02Z","timestamp":1731043622000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Analysis of Candidate\n                    <scp>miRNAs<\/scp>\n                    ' Expression in Pancreatic Cancer"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5662-7684","authenticated-orcid":false,"given":"Rabeah","family":"Al\u2010Temaimi","sequence":"first","affiliation":[{"name":"Human Genetics Unit, Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bicher","family":"Abdulkarim","sequence":"additional","affiliation":[{"name":"Undergraduate Medical Program, College of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Al\u2010Ali","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine Kuwait University  Jabriya Kuwait"},{"name":"Department of Gastroenterology and Hepatology Amiri Hospital  Kuwait City Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bency","family":"John","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mrinmay Kumar","family":"Mallik","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Mubarak Al Kabeer Hospital  Jabriya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kusum","family":"Kapila","sequence":"additional","affiliation":[{"name":"Department of Pathology, College of Medicine Kuwait University  Jabriya Kuwait"},{"name":"Department of Laboratory Medicine Mubarak Al Kabeer Hospital  Jabriya Kuwait"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,11,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21051723"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12938-021-00857-9"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/genes10100752"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21207691"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cancergen.2020.09.003"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2468-1253(19)30416-9"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-019-6284-y"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1210228"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00268-008-9833-0"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.4174\/astr.2014.87.6.290"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0087897"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10142-016-0512-1"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-05274-4"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12885-015-1785-9"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1080\/07357907.2022.2057526"},{"issue":"8","key":"e_1_2_9_18_1","article-title":"Clinical Value of Circulating microRNAs in Diagnosis and Prognosis of Pancreatic Cancer: A Systematic Review","volume":"15","author":"Senaratne M.","year":"2023","journal-title":"Cureus"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.19591"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-023-02865-2"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.1186\/s13000-015-0272-6"},{"issue":"6","key":"e_1_2_9_22_1","first-page":"3567","article-title":"MicroRNA\u2010217 Inhibits Cell Proliferation, Invasion and Migration by Targeting Tpd52l2 in Human Pancreatic Adenocarcinoma","volume":"38","author":"Chen Q.","year":"2017","journal-title":"Oncology Reports"},{"key":"e_1_2_9_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gendis.2022.12.018"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgq160"},{"key":"e_1_2_9_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2017.11.020"},{"key":"e_1_2_9_26_1","doi-asserted-by":"publisher","DOI":"10.2174\/156652412798376170"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.137364"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1159\/000493033"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2012.122"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tranon.2014.05.007"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.5114\/ceji.2016.65139"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1155\/2020\/6073150"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11081"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.70400","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,12,9]],"date-time":"2025-12-09T12:54:43Z","timestamp":1765284883000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.70400"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,11]]},"references-count":32,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/cam4.70400"],"URL":"https:\/\/doi.org\/10.1002\/cam4.70400","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,11]]},"assertion":[{"value":"2024-02-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-24","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-11-08","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70400"}}