{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:05:47Z","timestamp":1789585547695,"version":"build-2803163510"},"reference-count":59,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T00:00:00Z","timestamp":1708732800000},"content-version":"vor","delay-in-days":23,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["22ak0101187h0101"],"award-info":[{"award-number":["22ak0101187h0101"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Liver injury associated with oxaliplatin (L\u2010OHP)\u2010based chemotherapy can significantly impact the treatment outcomes of patients with colorectal cancer liver metastases, especially when combined with surgery. To date, no definitive biomarker that can predict the risk of liver injury has been identified. This study aimed to investigate whether organoids can be used as tools to predict the risk of liver injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      We examined the relationship between the clinical signs of L\u2010OHP\u2010induced liver injury and the responses of patient\u2010derived liver organoids\n                      <jats:italic>in vitro<\/jats:italic>\n                      . Organoids were established from noncancerous liver tissues obtained from 10 patients who underwent L\u2010OHP\u2010based chemotherapy and hepatectomy for colorectal cancer.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Organoids cultured in a galactose differentiation medium, which can activate the mitochondria of organoids, showed sensitivity to L\u2010OHP cytotoxicity, which was significantly related to clinical liver toxicity induced by L\u2010OHP treatment. Organoids from patients who presented with a high\u2010grade liver injury to the L\u2010OHP regimen showed an obvious increase in mitochondrial superoxide levels and a significant decrease in mitochondrial membrane potential with L\u2010OHP exposure. L\u2010OHP\u2010induced mitochondrial oxidative stress was not observed in the organoids from patients with low\u2010grade liver injury.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These results suggested that L\u2010OHP\u2010induced liver injury may be caused by mitochondrial oxidative damage. Furthermore, patient\u2010derived liver organoids may be used to assess susceptibility to L\u2010OHP\u2010induced liver injury in individual patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.7042","type":"journal-article","created":{"date-parts":[[2024,2,24]],"date-time":"2024-02-24T03:59:47Z","timestamp":1708747187000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Prediction for oxaliplatin\u2010induced liver injury using patient\u2010derived liver organoids"],"prefix":"10.1002","volume":"13","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-9300-183X","authenticated-orcid":false,"given":"Kumiko","family":"Tatsumi","sequence":"first","affiliation":[{"name":"Department of Cancer Drug Discovery and Development, Research Center Osaka International Cancer Institute  Osaka Japan"},{"name":"Department of Surgery, Graduate School of Medicine Kyoto University  Kyoto 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