{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T19:21:11Z","timestamp":1790882471820,"version":"4.1.0"},"reference-count":21,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,2,4]],"date-time":"2025-02-04T00:00:00Z","timestamp":1738627200000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,2]]},"abstract":"ABSTRACT<\/jats:title>Background<\/jats:title>Human nasopharyngeal carcinoma (NPC) cell lines are in\u00a0vitro model systems that are widely available, easy to handle, and provide an unlimited supply of material. They also bypass ethical concerns associated with the use of primary human cells or tissue. However, many of these cell lines including 5\u20108F, 6\u201010B, CNE\u20101, CNE\u20102, HNE\u20101, HONE\u20101, SUNE1, SUNE2, and NPC\u2010TW01 have been shown to be misidentified or cross\u2010contaminated. While simple molecular genotyping techniques such as short tandem repeat profiling of human cell lines are available to confirm cell line identity, scientists often do not implement strategies to avoid misidentification. This has resulted in a large volume of publications containing incorrect information.<\/jats:p><\/jats:sec>Methods<\/jats:title>In this paper, we have established a cell line karyogram that contains several marker chromosomes and a set of typical aberrations characteristic of NPC\/HK1.<\/jats:p><\/jats:sec>Results and Conclusions<\/jats:title>Combined with the typical multiloci short tandem repeat signature of NPC\/HK1, the cytogenetic analysis provides an effective means to avoid unreliable experimental outcomes and scientific misinterpretation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70422","type":"journal-article","created":{"date-parts":[[2025,2,5]],"date-time":"2025-02-05T06:40:26Z","timestamp":1738737626000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genetic Characterization of the Immortalized Human Nasopharyngeal Carcinoma Cell Line NPC<\/scp>\/HK1<\/scp>"],"prefix":"10.1002","volume":"14","author":[{"given":"Anna","family":"Makowska","sequence":"first","affiliation":[{"name":"Division of Pediatric Hematology, Oncology and Stem Cell Transplantation University Hospital Aachen Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Udo","family":"Kontny","sequence":"additional","affiliation":[{"name":"Division of Pediatric Hematology, Oncology and Stem Cell Transplantation University Hospital Aachen Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josef","family":"van Helden","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC) University Hospital Aachen Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Hildebrandt","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Herdit M.","family":"Sch\u00fcler","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics Medical Faculty and University Hospital D\u00fcsseldorf, Heinrich Heine University D\u00fcsseldorf Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3888-0931","authenticated-orcid":false,"given":"Ralf","family":"Weiskirchen","sequence":"additional","affiliation":[{"name":"Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry (IFMPEGKC) University Hospital Aachen Aachen Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3390\/cells13070559"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41416\u2010024\u201002675\u2010x"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.labinv.2024.102124"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910260202"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23374"},{"issue":"1","key":"e_1_2_11_7_1","first-page":"127","article-title":"Establishment of an Epitheloid Cell Line and a Fusiform Cell Line From a Patient With Nasopharyngeal Carcinoma","volume":"21","author":"Zeng Y.","year":"1978","journal-title":"Scientia Sinica"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910310509"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.23.9524"},{"issue":"6","key":"e_1_2_11_10_1","first-page":"713","article-title":"Establishment and Characterization of Two Nasopharyngeal Carcinoma Cell Lines","volume":"62","author":"Lin C. 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