{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,24]],"date-time":"2025-12-24T12:37:06Z","timestamp":1766579826524,"version":"3.41.2"},"reference-count":57,"publisher":"Wiley","issue":"23","license":[{"start":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T00:00:00Z","timestamp":1733875200000},"content-version":"vor","delay-in-days":10,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>Intraductal papillary mucinous neoplasms (IPMNs) are diverse premalignant tumors of the pancreas. They progress stepwise from adenoma to carcinoma and offer an opportunity for intervention prior to malignant transformation into pancreatic ductal adenocarcinoma (PDAC). The current study aimed to identify differentially expressed genes (DEGs) in invasive PDAC\u2010associated IPMN vs. noninvasive IPMN to understand the potential molecular changes involved in malignant transformation of IPMN into PDAC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Archived tissue and data from 12 patients with histologically proven invasive PDAC arising from IPMN specimens were assessed. Gene expression analysis was performed on RNA extracted from macro\u2010dissected tissue specimens using the NanoString nCounter PanCancer Progression assay. Statistical and pathway analysis was performed using SPSS v28 and Ingenuity Pathway Analysis, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 159 genes had significantly (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05, <jats:italic>q<\/jats:italic>\u2009&lt;\u20090.05) different expression in PDAC arising from IPMN compared with that from IPMN alone (91 overexpressed and 68 underexpressed). Interestingly, 14 of top 10 over\u2010 and underexpressed genes were predicted to translate secretory proteins, with SignalP scores approaching 1. A number of differential canonical pathways (e.g., LXR\/RXR activation pathway, glycolysis I gluconeogenesis I, and hepatic fibrosis) and potential upstream regulators (e.g., TGFB1, THBS2, etc.) were also identified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>A differential gene expression profile between PDAC arising from IPMN and IPMN alone was identified. Pathway analysis identified potential mechanisms involved in malignant transformation of IPMN to PDAC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70499","type":"journal-article","created":{"date-parts":[[2024,12,11]],"date-time":"2024-12-11T17:41:34Z","timestamp":1733938894000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Gene Expression Profiling of Pancreatic Ductal Adenocarcinoma Arising From Intraductal Papillary Mucinous Neoplasms of the Pancreas"],"prefix":"10.1002","volume":"13","author":[{"given":"William A.","family":"Ziaziaris","sequence":"first","affiliation":[{"name":"Department of Upper Gastrointestinal Surgery Royal North Shore Hospital  Sydney Australia"},{"name":"Faculty of Medical and Health The University of Sydney  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher S. H.","family":"Lim","sequence":"additional","affiliation":[{"name":"Department of Upper Gastrointestinal Surgery Royal North Shore Hospital  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Loretta","family":"Sioson","sequence":"additional","affiliation":[{"name":"NSW Health Pathology, Department of Anatomical Pathology Royal North Shore Hospital  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony J.","family":"Gill","sequence":"additional","affiliation":[{"name":"Faculty of Medical and Health The University of Sydney  Sydney Australia"},{"name":"NSW Health Pathology, Department of Anatomical Pathology Royal North Shore Hospital  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaswinder S.","family":"Samra","sequence":"additional","affiliation":[{"name":"Department of Upper Gastrointestinal Surgery Royal North Shore Hospital  Sydney Australia"},{"name":"Faculty of Medical and Health The University of Sydney  Sydney Australia"},{"name":"Australian Pancreatic Centre  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2900-8845","authenticated-orcid":false,"given":"Sumit","family":"Sahni","sequence":"additional","affiliation":[{"name":"Faculty of Medical and Health The University of Sydney  Sydney Australia"},{"name":"Australian Pancreatic Centre  Sydney Australia"},{"name":"Kolling Institute of Medical Research The University of Sydney  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anubhav","family":"Mittal","sequence":"additional","affiliation":[{"name":"Department of Upper Gastrointestinal Surgery Royal North Shore Hospital  Sydney Australia"},{"name":"Faculty of Medical and Health The University of Sydney  Sydney Australia"},{"name":"Australian Pancreatic Centre  Sydney Australia"},{"name":"School of Medicine The University of Notre Dame  Sydney Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,12,11]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_10_2_1","DOI":"10.1007\/s00428\u2010005\u20100039\u20107"},{"doi-asserted-by":"publisher","key":"e_1_2_10_3_1","DOI":"10.1007\/s00428\u2010007\u20100512\u20106"},{"doi-asserted-by":"publisher","key":"e_1_2_10_4_1","DOI":"10.1007\/s00535\u2010005\u20101646\u20104"},{"doi-asserted-by":"publisher","key":"e_1_2_10_5_1","DOI":"10.1016\/j.pan.2017.07.007"},{"doi-asserted-by":"publisher","key":"e_1_2_10_6_1","DOI":"10.1007\/s00330\u2010020\u201007583\u20101"},{"doi-asserted-by":"publisher","key":"e_1_2_10_7_1","DOI":"10.1053\/j.gastro.2015.07.041"},{"doi-asserted-by":"publisher","key":"e_1_2_10_8_1","DOI":"10.1007\/s11605\u2010009\u20100936\u20101"},{"doi-asserted-by":"publisher","key":"e_1_2_10_9_1","DOI":"10.1158\/1055\u20109965.EPI\u201018\u20100483"},{"doi-asserted-by":"publisher","key":"e_1_2_10_10_1","DOI":"10.1016\/S0002\u20109440(10)63178\u20101"},{"doi-asserted-by":"publisher","key":"e_1_2_10_11_1","DOI":"10.1186\/s10020\u2010022\u201000467\u20108"},{"doi-asserted-by":"publisher","key":"e_1_2_10_12_1","DOI":"10.18388\/ABP.2020_5225"},{"doi-asserted-by":"publisher","key":"e_1_2_10_13_1","DOI":"10.1016\/j.ejso.2020.02.001"},{"doi-asserted-by":"publisher","key":"e_1_2_10_14_1","DOI":"10.3389\/fonc.2020.00237"},{"doi-asserted-by":"publisher","key":"e_1_2_10_15_1","DOI":"10.1038\/s41587\u2010021\u201001156\u20103"},{"doi-asserted-by":"publisher","key":"e_1_2_10_16_1","DOI":"10.1002\/path.2250"},{"doi-asserted-by":"publisher","key":"e_1_2_10_17_1","DOI":"10.1053\/j.gastro.2009.04.009"},{"doi-asserted-by":"publisher","key":"e_1_2_10_18_1","DOI":"10.3389\/fonc.2021.708963"},{"doi-asserted-by":"publisher","key":"e_1_2_10_19_1","DOI":"10.3390\/ijms23105459"},{"doi-asserted-by":"publisher","key":"e_1_2_10_20_1","DOI":"10.1097\/01.sla.0000154455.86404.e9"},{"doi-asserted-by":"publisher","key":"e_1_2_10_21_1","DOI":"10.1080\/2162402X.2021.2008110"},{"doi-asserted-by":"publisher","key":"e_1_2_10_22_1","DOI":"10.6092\/1590\u20108577\/3330"},{"doi-asserted-by":"publisher","key":"e_1_2_10_23_1","DOI":"10.1158\/1078\u20100432.CCR\u201012\u20102662"},{"doi-asserted-by":"publisher","key":"e_1_2_10_24_1","DOI":"10.1158\/2159\u20108290.CD\u201022\u20101200"},{"doi-asserted-by":"publisher","key":"e_1_2_10_25_1","DOI":"10.1016\/j.humpath.2017.03.008"},{"doi-asserted-by":"publisher","key":"e_1_2_10_26_1","DOI":"10.1093\/ajcp\/aqy030"},{"doi-asserted-by":"publisher","key":"e_1_2_10_27_1","DOI":"10.3390\/cancers11081068"},{"doi-asserted-by":"publisher","key":"e_1_2_10_28_1","DOI":"10.4143\/crt.2016.550"},{"doi-asserted-by":"publisher","key":"e_1_2_10_29_1","DOI":"10.1373\/clinchem.2005.065722"},{"doi-asserted-by":"publisher","key":"e_1_2_10_30_1","DOI":"10.1002\/elps.201200583"},{"doi-asserted-by":"publisher","key":"e_1_2_10_31_1","DOI":"10.1016\/j.cell.2017.12.026"},{"doi-asserted-by":"publisher","key":"e_1_2_10_32_1","DOI":"10.1038\/ncb3340"},{"doi-asserted-by":"publisher","key":"e_1_2_10_33_1","DOI":"10.1136\/gutjnl\u20102020\u2010323014"},{"doi-asserted-by":"publisher","key":"e_1_2_10_34_1","DOI":"10.1038\/nrgastro.2012.115"},{"doi-asserted-by":"publisher","key":"e_1_2_10_35_1","DOI":"10.1016\/j.neo.2022.100789"},{"unstructured":"United States Library of Medicine \u201cChemotherapy + Anakinra in Patients With Pancreatic Adenocarcinoma (PDAC) \u201d (2021) https:\/\/clinicaltrials.gov\/ct2\/show\/NCT04926467.","key":"e_1_2_10_36_1"},{"unstructured":"United States Library of Medicine \u201cStudy of Onivyde and 5\u2010FU in Combination With Xilonix for Pancreatic Cancer With Cachexia (OnFX) \u201d (2020) https:\/\/clinicaltrials.gov\/ct2\/show\/NCT03207724.","key":"e_1_2_10_37_1"},{"doi-asserted-by":"publisher","key":"e_1_2_10_38_1","DOI":"10.1158\/0008\u20105472.CAN\u201012\u20100634"},{"doi-asserted-by":"publisher","key":"e_1_2_10_39_1","DOI":"10.1186\/s12964\u2010017\u20100175\u20100"},{"doi-asserted-by":"publisher","key":"e_1_2_10_40_1","DOI":"10.1593\/neo.81618"},{"doi-asserted-by":"publisher","key":"e_1_2_10_41_1","DOI":"10.1016\/S0304\u20103835(03)00012\u20100"},{"doi-asserted-by":"publisher","key":"e_1_2_10_42_1","DOI":"10.1016\/S0360\u20103016(00)00803\u20108"},{"doi-asserted-by":"publisher","key":"e_1_2_10_43_1","DOI":"10.1016\/j.ymthe.2021.01.002"},{"doi-asserted-by":"publisher","key":"e_1_2_10_44_1","DOI":"10.1001\/jamaoncol.2019.0892"},{"doi-asserted-by":"publisher","key":"e_1_2_10_45_1","DOI":"10.1038\/nrd1580"},{"unstructured":"United States Library of Medicine \u201cSafety and Tolerability of AP 12009 Administered I.V. in Patients With Advanced Tumors Known to Overproduce TGF\u2010Beta\u20102 \u201d (2019) https:\/\/clinicaltrials.gov\/ct2\/show\/NCT00844064.","key":"e_1_2_10_46_1"},{"doi-asserted-by":"publisher","key":"e_1_2_10_47_1","DOI":"10.1177\/1758835920917558"},{"doi-asserted-by":"publisher","key":"e_1_2_10_48_1","DOI":"10.1042\/CS20180816"},{"key":"e_1_2_10_49_1","first-page":"2618","article-title":"DcR3 Promotes Proliferation and Invasion of Pancreatic Cancer via a DcR3\/STAT1\/IRF1 Feedback Loop","volume":"9","author":"Wei Y.","year":"2019","journal-title":"American Journal of Cancer Research"},{"doi-asserted-by":"publisher","key":"e_1_2_10_50_1","DOI":"10.18632\/oncotarget.3286"},{"doi-asserted-by":"publisher","key":"e_1_2_10_51_1","DOI":"10.3892\/or.2018.6701"},{"doi-asserted-by":"publisher","key":"e_1_2_10_52_1","DOI":"10.1097\/MPA.0000000000000074"},{"doi-asserted-by":"publisher","key":"e_1_2_10_53_1","DOI":"10.1089\/jir.2014.0157"},{"doi-asserted-by":"publisher","key":"e_1_2_10_54_1","DOI":"10.1126\/sciadv.ade4582"},{"doi-asserted-by":"publisher","key":"e_1_2_10_55_1","DOI":"10.1038\/s41467\u2010024\u201046994\u20102"},{"doi-asserted-by":"publisher","key":"e_1_2_10_56_1","DOI":"10.1016\/j.ejso.2018.10.050"},{"doi-asserted-by":"publisher","key":"e_1_2_10_57_1","DOI":"10.1042\/bst0311243"},{"doi-asserted-by":"publisher","key":"e_1_2_10_58_1","DOI":"10.1016\/S0962\u20108924(98)01360\u20109"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.70499","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,19]],"date-time":"2025-02-19T09:55:54Z","timestamp":1739958954000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.70499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12]]},"references-count":57,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2024,12]]}},"alternative-id":["10.1002\/cam4.70499"],"URL":"https:\/\/doi.org\/10.1002\/cam4.70499","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"type":"print","value":"2045-7634"},{"type":"electronic","value":"2045-7634"}],"subject":[],"published":{"date-parts":[[2024,12]]},"assertion":[{"value":"2023-12-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-12-11","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70499"}}