{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T12:45:35Z","timestamp":1791549935247,"version":"4.3.4"},"reference-count":26,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,3,10]],"date-time":"2025-03-10T00:00:00Z","timestamp":1741564800000},"content-version":"vor","delay-in-days":9,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100022274","name":"Daiichi Sankyo Europe","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100022274","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Triple\u2010Negative Breast Cancer (TNBC) is an aggressive breast cancer subtype, in which targeting the Trophoblast cell\u2010surface antigen\u20102 (Trop\u20102), using antibody\u2010drug conjugates (ADC), results in significant clinical improvement. However, clinicopathological correlations with Trop\u20102 protein expression levels remain limited in TNBC patients.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Here we assessed by immunohistochemistry (IHC) using the mouse monoclonal anti\u2010Trop\u20102 antibody (Enzo, Cat. ENZ\u2010ABS380) cell membrane Trop\u20102 expression levels and classified them in 3 H\u2010Score classes, low (&lt;\u2009100), moderate (100\u2013200), and strong (&gt;\u2009200). We also evaluated potential associations with clinicopathological variables including basal\u2010like and molecular apocrine phenotypes, immune infiltrate characteristics,\n                      <jats:italic>PTEN<\/jats:italic>\n                      and\n                      <jats:italic>PIK3CA<\/jats:italic>\n                      alterations in a large retrospective series of 228 nonmetastatic TNBC patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Trop\u20102 expression was evaluated as low, moderate and strong in 12.3%, 28.9%, and 58.8% of the cases respectively. Only 3 tumors showed no Trop\u20102 expression. Interestingly, Trop\u20102 expression was not associated with classical breast cancer clinicopathological variables, HER2 levels or molecular subtype, neither did we observe an association with relapse\u2010free survival. Only a marginal association with pT1 tumors was observed, which tended to express increased levels of Trop\u20102 protein.<\/jats:p>\n                    <jats:p>In order to determine possible fluctuations of Trop\u20102 protein expression levels during the course of the disease, we studied a second independent cohort of 18 TNBC comprised of serial tissue samples (diagnostic biopsies, surgical resection specimens and corresponding patients\u2010derived xenografts (PDX)). Trop\u20102 levels remained globally stable between cognate tumor samples with only one exception corresponding to a Trop\u20102\u2010negative tumor giving rise to a Trop\u20102\u2010positive PDX.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>As Trop\u20102 expression appears nearly constant and independent of classical TNBC variables and outcome, association of anti\u2010Trop\u20102 therapies with other targeted therapies can be evaluated without reducing the population in specific TNBC subgroups.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.70615","type":"journal-article","created":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T04:11:49Z","timestamp":1741752709000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Prognostic Value of Trop\u20102 Expression in Nonmetastatic Triple\u2010Negative Breast Cancer and Correlation With Emerging Biomarkers"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7834-061X","authenticated-orcid":false,"given":"William","family":"Jacot","sequence":"first","affiliation":[{"name":"Department of Medical Oncology Montpellier Cancer Institute Val d'Aurelle  Montpellier France"},{"name":"Translational Research Unit Montpellier Cancer Institute Val d'Aurelle  Montpellier France"},{"name":"Montpellier University  Montpellier France"},{"name":"Institut de Recherche en Canc\u00e9rologie de Montpellier (IRCM)  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie\u2010Christine","family":"Chateau","sequence":"additional","affiliation":[{"name":"Translational Research Unit Montpellier Cancer Institute Val d'Aurelle  Montpellier 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