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To preliminarily explore the feasibility of cancer vaccines based on cancer testis antigen in the immunotherapy of sarcomas, we investigate the expression of Cancer\/Testis Antigens (CTA) MAGE\u2010A4, PRAME, MAGE\u2010A1, KK\u2010LC\u20101, and NY\u2010ESO\u20101 in bone and soft tissue sarcomas, with the aim of assessing their potential for use in sarcoma immunotherapy and determining their expression levels in different subtypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and Results<\/jats:title><jats:p>We employed immunohistochemistry and multiplex immunostaining microarrays (MI chips) to assess the expression of MAGE\u2010A4, PRAME, MAGE\u2010A1, KK\u2010LC\u20101, and NY\u2010ESO\u20101 in 21 cases of undifferentiated pleomorphic sarcoma (UPS), 26 cases of smooth muscle sarcoma, 28 cases of liposarcoma, 40 cases of osteosarcoma (OS), and 13 cases of chondrosarcoma. MAGE\u2010A1 showed the highest expression in osteosarcoma (32.50%), while it was lower in liposarcoma and undifferentiated pleomorphic sarcoma (10.71% and 10.00%) and undetectable in chondrosarcoma. MAGE\u2010A4 expression was elevated in osteosarcoma and undifferentiated pleomorphic sarcoma (40.00% and 33.00%), but lower in liposarcoma and smooth muscle sarcoma (17.00% and 33.00%). NY\u2010ESO\u20101 expression was relatively low across all sarcoma subtypes. PRAME expression was highest in undifferentiated pleomorphic sarcoma (47.62%) and low in chondrosarcoma (7.69%). None of the sarcomas expressed KK\u2010LC\u20101. Additionally, while there was no statistically significant correlation between CTA expression and patient age or gender, some differences related to age and gender were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>CTA expression in bone and soft tissue sarcomas was correlated with both CTA type and sarcoma subtype, showing relatively high levels of expression in undifferentiated pleomorphic sarcoma (UPS) and osteosarcoma (OS). The poly\u2010expression of MAGE\u2010A4, PRAME, and MAGE\u2010A1 across all subtypes suggests that these antigens may serve as potential targets for sarcoma\u2010specific immunotherapy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70750","type":"journal-article","created":{"date-parts":[[2025,3,30]],"date-time":"2025-03-30T23:05:59Z","timestamp":1743375959000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression of Cancer\u2010Testis Antigens <scp>MAGE<\/scp>\u2010<scp>A1<\/scp>, <scp>MAGE<\/scp>\u2010<scp>A4<\/scp>, <scp>NY<\/scp>\u2010<scp>ESO<\/scp>\u20101 and <scp>PRAME<\/scp> in Bone and Soft Tissue Sarcomas: The Experience From a Single Center in China"],"prefix":"10.1002","volume":"14","author":[{"given":"Anni","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Oncology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University  Nanjing 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