{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T05:26:13Z","timestamp":1783488373721,"version":"3.55.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2025,3,25]],"date-time":"2025-03-25T00:00:00Z","timestamp":1742860800000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Differentiated thyroid cancer (DTC) is the primary subtype of thyroid cancer. Timely diagnosis and intervention are crucial for improving prognosis and survival. However, the effectiveness of existing serum markers is limited, necessitating the discovery of new biomarkers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study utilized liquid chromatography\u2013tandem mass spectrometry to analyze tryptophan metabolic profiles in serum samples from 105 DTC patients and 50 healthy controls. Independent predictors of DTC were identified through univariate intergroup comparisons and multivariate logistic regression analysis, leading to the development and validation of a new diagnostic model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significant differences were observed in 11 tryptophan metabolites between DTC patients and controls. Logistic regression identified nicotinamide, 3\u2010hydroxyanthranilic acid, 5\u2010hydroxytryptophan, melatonin, and indole\u20103\u2010propionic acid as independent predictors. The nomogram prediction model was established based on these five metabolites, and according to the Hosmer\u2013Lemeshow test, the model showed good fit. The five\u2010metabolite diagnostic model demonstrated 84.8% sensitivity, 90.0% specificity, and an area under the ROC curve of 0.932. Decision curve analysis indicated that the model had significant advantages over serum thyroglobulin.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>Tryptophan metabolism exhibits distinct changes in DTC patients, with specific metabolites serving as early diagnostic markers. The five\u2010metabolite panel demonstrates potential for improving early detection and management of DTC.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70808","type":"journal-article","created":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T04:46:04Z","timestamp":1743050764000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Analysis of Tryptophan Metabolic Profile Characteristics and Clinical Value in Differentiated Thyroid Cancer Patients"],"prefix":"10.1002","volume":"14","author":[{"given":"Qiang","family":"Miao","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinhua","family":"Dai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Luo","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9364-1581","authenticated-orcid":false,"given":"Junlong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9674-0139","authenticated-orcid":false,"given":"Han","family":"Luo","sequence":"additional","affiliation":[{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"},{"name":"Division of Thyroid and Parathyroid Surgery West China Hospital, Sichuan University  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2597-4915","authenticated-orcid":false,"given":"Bei","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine West China Hospital, Sichuan University  Chengdu Sichuan China"},{"name":"Sichuan Clinical Research Center for Laboratory Medicine  Chengdu Sichuan China"},{"name":"Clinical Laboratory Medicine Research Center of West China Hospital  Chengdu Sichuan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,3,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.2015.0020"},{"key":"e_1_2_11_3_1","unstructured":"Society A. C \u201cWhat Is Thyroid Cancer? \u201d2024 https:\/\/www.cancer.org\/cancer\/types\/thyroid\u2010cancer\/about\/what\u2010is\u2010thyroid\u2010cancer.html."},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21708"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jncc.2024.01.006"},{"issue":"7","key":"e_1_2_11_7_1","first-page":"325","article-title":"China Anti\u2010Cancer Association Guidelines for Holistic Integrative Management of Thyroid Cancer (2022 Abridged Version)","volume":"50","author":"China Anti\u2010Cancer Association Committee of Thyroid Oncology","year":"2023","journal-title":"Chinese Journal of Clinical Oncology"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.beem.2022.101687"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(23)00020-X"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.4158\/EP12047.CO"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15102692"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(18)30127-X"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/cac2.12197"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2023.26348"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/dju097"},{"issue":"3","key":"e_1_2_11_16_1","first-page":"518","article-title":"Thyroglobulin as a Tumor Marker in Differentiated Thyroid Cancer \u2010 Clinical Considerations","volume":"57","author":"Prpic M.","year":"2018","journal-title":"Acta Clinica Croatica"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21670"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2023.06.004"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-002840"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202204006"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2021.593510"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25063495"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms21155272"},{"issue":"4","key":"e_1_2_11_24_1","first-page":"531","article-title":"The Present Situation and Prospect of Metabolomics in the Diagnosis and Treatment of Thyroid Follicular Tumors","volume":"57","author":"Qin J. L.","year":"2022","journal-title":"Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21439"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60071080"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.3390\/medicina60091445"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.7189\/jogh.14.04131"},{"issue":"6","key":"e_1_2_11_29_1","first-page":"917","article-title":"Analysis and Prediction of Thyroid Cancer Morbidity and Mortality Trends in China","volume":"44","author":"Yao C. Z.","year":"2023","journal-title":"Zhonghua Liu Xing Bing Xue Za Zhi"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2023.1156999"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000036426"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12967-019-1984-2"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/bmc.3773"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.1158\/1538-7445.AM2023-1004"},{"key":"e_1_2_11_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202207074"},{"key":"e_1_2_11_36_1","doi-asserted-by":"publisher","DOI":"10.1111\/apha.12430"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13215311"},{"key":"e_1_2_11_38_1","doi-asserted-by":"publisher","DOI":"10.1111\/jpi.12693"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.32794\/mr11250094"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-4996-z"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmet.2024.05.013"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1021\/acschemneuro.2c00418"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25063389"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2023.41.16_suppl.e15523"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xcrm.2023.101061"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep30075"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.1530\/ERC-19-0344"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2021.122729"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.70808","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,27]],"date-time":"2025-05-27T12:19:50Z","timestamp":1748348390000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.70808"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2025,3]]}},"alternative-id":["10.1002\/cam4.70808"],"URL":"https:\/\/doi.org\/10.1002\/cam4.70808","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,3]]},"assertion":[{"value":"2024-12-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-12","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-25","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70808"}}