{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T22:19:53Z","timestamp":1791843593788,"version":"4.3.4"},"reference-count":65,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,5]],"date-time":"2025-05-05T00:00:00Z","timestamp":1746403200000},"content-version":"vor","delay-in-days":4,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001502","name":"Department of Atomic Energy, Government of India","doi-asserted-by":"publisher","award":["RIN\u20104002\u2010SBS"],"award-info":[{"award-number":["RIN\u20104002\u2010SBS"]}],"id":[{"id":"10.13039\/501100001502","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>The gastric epithelium experiences intermittent hypoxia due to various physiological and pathological conditions. However, the impact of hypoxia and hypoxia\u2010reoxygenation of gastric epithelial cells (GECs) on Helicobacter pylori\u2010mediated gastric cancer (GC) has never been investigated. Carcinoembryonic antigen\u2010related cell adhesion molecules (CEACAMs) facilitate H.\u2009pylori adhesion onto GECs. We evaluated the effect of hypoxia and hypoxia\u2010reoxygenation on CEACAM6\u2010mediated H.\u2009pylori binding, infection, reactive oxygen species (ROS) generation, and GEC proliferation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Hypoxia\u2010inducible factor 1 (HIF1\u03b1) and CEACAM6 levels were assessed in various GECs. ROS were measured using 2',7'\u2010dichlorofluorescin diacetate (DCFDA). Bioinformatics analyses were performed to identify the most prominent stomach adenocarcinoma (STAD)\u2010associated NADPH oxidase (NOX) followed by validation by overexpression\/suppression studies and western blotting. GC biopsies were examined by immunofluorescence microscopy. Hypoxia\u2010exposed, reoxygenated, or control cells were compared for ROS generation and H.\u2009pylori infection. MTT assay determined cell proliferation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results and Conclusions<\/jats:title>\n                    <jats:p>Hypoxia and HIF1 mediated upregulation of CEACAM6 in GECs. CEACAM6 significantly promoted ROS generation by inducing NOX4 in hypoxic GECs. HIF1\u03b1, CEACAM6, and NOX4 upregulation was detected in gastritis and GC tissues. H.\u2009pylori infection significantly increased in hypoxia\u2010exposed GECs as compared to normoxic GECs. Infection of hypoxia\u2010reoxygenated GECs also resulted in significantly increased CEACAM6 and NOX4\u2010mediated ROS generation compared to normoxic GECs. In addition, adhesion of H.\u2009pylori, cytotoxin\u2010associated gene A (CagA) translocation, and GEC proliferation were significantly enhanced in hypoxia\u2010reoxygenated GECs. Collectively, this study established that hypoxia and hypoxia\u2010reoxygenation of GECs facilitate H.\u2009pylori infection and infection\u2010mediated GEC proliferation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.70860","type":"journal-article","created":{"date-parts":[[2025,5,6]],"date-time":"2025-05-06T01:39:15Z","timestamp":1746495555000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Hypoxia and Hypoxia\u2010Reoxygenation Potentiate\n                    <scp>\n                      <i>Helicobacter pylori<\/i>\n                    <\/scp>\n                    Infection and Gastric Epithelial Cell Proliferation"],"prefix":"10.1002","volume":"14","author":[{"given":"Indrajit","family":"Poirah","sequence":"first","affiliation":[{"name":"School of Biological Sciences, National Institute of Science Education and Research (NISER) Bhubaneswar An OCC of Homi Bhabha National Institute  Odisha 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