{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,15]],"date-time":"2026-02-15T08:04:04Z","timestamp":1771142644250,"version":"3.50.1"},"reference-count":39,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T00:00:00Z","timestamp":1746662400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>\n                      To investigate the clinical characteristics and prognosis of mutation spots and concomitant gene mutations in myelodysplastic syndromes (MDS) with\n                      <jats:italic>SF3B1<\/jats:italic>\n                      mutation (\n                      <jats:italic>SF3B1<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>mut<\/jats:italic>\n                      <\/jats:sup>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and Methods<\/jats:title>\n                    <jats:p>\n                      Patients diagnosed with MDS at Shanghai Jiao Tong University School of Medicine Affiliated Sixth People's Hospital from October 2008 to November 2023 were enrolled in this study.\n                      <jats:italic>SF3B1<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>mut<\/jats:italic>\n                      <\/jats:sup>\n                      was identified by next\u2010generation sequencing (NGS).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      One hundred and seven (8.7%) cases harbored the\n                      <jats:italic>SF3B1<\/jats:italic>\n                      mutation. The most frequent\n                      <jats:italic>SF3B1<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>mut<\/jats:italic>\n                      <\/jats:sup>\n                      , noted in 47.66% of all patients, was the hotspot\n                      <jats:italic>K700E<\/jats:italic>\n                      .\n                      <jats:italic>K666<\/jats:italic>\n                      and\n                      <jats:italic>R625<\/jats:italic>\n                      were observed in 24.30% and 9.35%, respectively. Two less frequent mutation subtypes accounted for 5.61% of\n                      <jats:italic>H662<\/jats:italic>\n                      and 4.67% of\n                      <jats:italic>E622<\/jats:italic>\n                      . Patients with the\n                      <jats:italic>K666<\/jats:italic>\n                      mutation showed more severe thrombocytopenia (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.032), significantly lower NK cell percentage (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.001), and the Th1\/Th2 ratio (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.018) in the bone marrow (BM). The overall survival (OS) in patients with\n                      <jats:italic>E622<\/jats:italic>\n                      and\n                      <jats:italic>H662<\/jats:italic>\n                      mutations was significantly longer than that of patients with the\n                      <jats:italic>R625<\/jats:italic>\n                      mutation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.045) and the\n                      <jats:italic>K666<\/jats:italic>\n                      mutation (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.010). Multi\u2010variance analysis showed the\n                      <jats:italic>SF3B1<\/jats:italic>\n                      mutation involving the\n                      <jats:italic>K666<\/jats:italic>\n                      hotspot independently predicted overall survival in MDS (HR 2.094,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.050). Notably, most (11\/13, 84.6%) of concomitant\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations were mono\u2010hit, which did not affect the survival of patients in our cohort.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      <jats:italic>SF3B1<\/jats:italic>\n                      <jats:sup>\n                        <jats:italic>mut<\/jats:italic>\n                      <\/jats:sup>\n                      patients with specific mutation spots and concomitant gene mutations showed distinct clinical features and prognosis. Consequently, a comprehensive study of specific subtypes is of great significance for improving the prognosis of patients with\n                      <jats:italic>SF3B1<\/jats:italic>\n                      mutations.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.70930","type":"journal-article","created":{"date-parts":[[2025,5,8]],"date-time":"2025-05-08T07:25:23Z","timestamp":1746689123000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Significance of Mutation Spots and Concurrent Gene Mutations on Prognosis and Clinical Outcomes in Myelodysplastic Syndromes With\n                    <scp>\n                      <i>SF3B1<\/i>\n                    <\/scp>\n                    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