{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:11:59Z","timestamp":1790611919003,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T00:00:00Z","timestamp":1747785600000},"content-version":"vor","delay-in-days":20,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100017623","name":"Zhejiang Provincial Outstanding Youth Science Foundation","doi-asserted-by":"publisher","award":["LQ19H160038"],"award-info":[{"award-number":["LQ19H160038"]}],"id":[{"id":"10.13039\/501100017623","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100020734","name":"Zhejiang Provincial Research Center for Cancer Intelligent Diagnosis and Molecular Technology","doi-asserted-by":"publisher","award":["JBZX\u2010202003"],"award-info":[{"award-number":["JBZX\u2010202003"]}],"id":[{"id":"10.13039\/100020734","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Tertiary lymphoid structure (TLS) has been reported to be associated with prognosis and immunotherapy in certain cancers. The objective of our study was to investigate the prognostic significance of Tertiary Lymphoid Structures (TLS) within the context of Muscle\u2010Invasive Bladder Cancer (MIBC), while concurrently examining the clinicopathological and molecular determinants influencing TLS formation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemistry was used to detect the expression of TLS, CD8+ T cells, B cells, and plasma cells in 119 MIBC cases, of which 80 cases were tested by next generation sequencing (NGS) for analyzing the differences in gene alterations between TLS\u2010negative and TLS\u2010positive.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      TLS were identified in 52.1% (62\/119) of the MIBC cases studied. Patients exhibiting TLS demonstrated reduced T and TNM staging and prolonged overall survival (OS) compared to those lacking TLS. Multivariate analysis showed that TLS was an independent prognostic factor. Densities of B cells, CD8+ T cells, and plasma cells in tumors were significantly correlated with TLS, but in the cases with low\u2010density B cells, high\u2010density CD8+ T cells, or high\u2010density plasma cells, differences in OS between the tumors with TLS and without TLS were not significant. Compared with TLS\u2010negative tumors, TLS\u2010positive tumors had a lower frequency of\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations and higher frequencies of\n                      <jats:italic>FAT1<\/jats:italic>\n                      and\n                      <jats:italic>CDKN1A<\/jats:italic>\n                      mutations. Tumor mutational burden (TMB) was not significantly different between the two groups but was significantly associated with TLS in\n                      <jats:italic>TP53<\/jats:italic>\n                      wild\u2010type tumors.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      TLS emerged as an independent harbinger of favorable prognosis in MIBC, predominantly mediating antitumor responses via B cells. Moreover,\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations were identified as a potential inhibitor of TLS formation.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.70978","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T06:24:25Z","timestamp":1747808665000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Tertiary Lymphoid Structures as Independent Predictors of Favorable Prognosis in Muscle\u2010Invasive Bladder Cancer"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8700-4770","authenticated-orcid":false,"given":"Xiaodong","family":"Teng","sequence":"first","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanfeng","family":"Bai","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liming","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pathology, the First Affiliated Hospital Zhejiang University School of Medicine  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaihua","family":"Liu","sequence":"additional","affiliation":[{"name":"Nanjing Geneseeq Technology Inc.  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuqian","family":"Shi","sequence":"additional","affiliation":[{"name":"Nanjing Geneseeq Technology Inc.  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yang","family":"Shao","sequence":"additional","affiliation":[{"name":"Nanjing Geneseeq Technology Inc.  Nanjing Jiangsu China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.17598"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abf9419"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41568-024-00728-0"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2024.112790"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.euo.2023.03.002"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.3233\/BLC-170120"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1136\/jitc-2021-003883"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-021-00232-6"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(18)31257-1"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.08.061"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.12.028"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaa4971"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.3889\/oamjms.2018.341"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-021-02242-x"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.annonc.2022.06.007"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijms.56347"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2020.11828"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1378844"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201309-1611OC"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2762"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.20892\/j.issn.2095-3941.2021.0029"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2832"},{"key":"e_1_2_12_24_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2016.00401"},{"key":"e_1_2_12_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2019.1583547"},{"key":"e_1_2_12_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.01.021"},{"key":"e_1_2_12_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2017.08.028"},{"key":"e_1_2_12_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2017.11.005"},{"key":"e_1_2_12_29_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.27287"},{"key":"e_1_2_12_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dnarep.2020.102785"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.70978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,28]],"date-time":"2025-05-28T06:32:31Z","timestamp":1748413951000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.70978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/cam4.70978"],"URL":"https:\/\/doi.org\/10.1002\/cam4.70978","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5]]},"assertion":[{"value":"2024-05-05","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-11","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-05-21","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70978"}}