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Recent studies have found that gut probiotics and their metabolic products play a significant role in activating the immune system, reshaping the tumor microenvironment, and inhibiting cancer progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We established a 4T1 tumor\u2010bearing mice model and analyzed the proportions of CD4<jats:sup>+<\/jats:sup> T and CD8<jats:sup>+<\/jats:sup> T cells in the spleen using flow cytometry and immunohistochemistry. The expression levels of TNF\u2010\u03b1, IL\u20106, and IL\u201010 were measured by enzyme\u2010linked immunosorbent assay. Hematoxylin\u2013eosin staining was used to observe the tumor morphology. Selective protein blotting and quantitative real\u2010time PCR were used to analyze the expression of Bax, Bcl\u20102, and Caspase\u20103. Cell proliferation was evaluated using the MTT assay, and apoptosis was detected by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results indicated that oral administration of CB and AKK possesses the capability to inhibit the progression of 4T1 breast cancer; however, the combined treatment with both strains (CB\u2010AKK) exhibited significantly superior effects compared to each individual strain. Further mechanistic analysis revealed that the CB\u2010AKK combination could activate the antitumor immunity in mice and reshape the tumor microenvironment. Additionally, it was found that the live bacteria and their metabolites derived from CB\u2010AKK could inhibit cell proliferation and promote tumor apoptosis by activating the Bcl\u20102\/Bax signaling pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study is the first to demonstrate that orally administered live bacteria CB\u2010AKK can inhibit the progression of 4T1 breast cancer, providing a promising new strategy for the development of innovative biotherapies for breast cancer.<\/jats:p><\/jats:sec>","DOI":"10.1002\/cam4.70987","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T10:48:48Z","timestamp":1749725328000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Oral Combined Probiotics <i>Clostridium butyricum<\/i> and <i>Akkermansia muciniphila<\/i> Inhibits the Progression of <scp>4T1<\/scp> Breast Cancer by Activating Bcl\u20102\/Bax Pathway"],"prefix":"10.1002","volume":"14","author":[{"given":"Xiaowei","family":"Li","sequence":"first","affiliation":[{"name":"Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou &amp; 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