{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T23:07:29Z","timestamp":1792192049751,"version":"4.4.0"},"reference-count":41,"publisher":"Wiley","issue":"21","license":[{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,10,29]],"date-time":"2025-10-29T00:00:00Z","timestamp":1761696000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>BUB1 mitotic checkpoint serine\/threonine kinase B (BUB1B) has been found to participate in cancer progression. Nevertheless, the function and mechanism of BUB1B in ovarian cancer (OC) remain unknown.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Method<\/jats:title>\n                    <jats:p>\n                      Based on datasets GSE14407, GSE18520, and the TCGA combined GTEx database, the differently expressed genes (DEGs) between OC tissues and para\u2010carcinoma tissues were identified. These DEGs were subjected to protein\u2013protein interaction analysis to obtain hub genes, with BUB1B serving as a candidate for further study. Subsequently, the prognostic value was analyzed. To elucidate the role of BUB1B, knockdown experiments were conducted in\u00a0vitro and in\u00a0vivo to assess alterations in malignant behaviors, while\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin expression was quantified by qRT\u2010PCR and western blot. Moreover, the Wnt\/\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin pathway inhibitor LGK974 was utilized to demonstrate whether the effects of BUB1B are mediated by the Wnt\/\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin pathway.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      High BUB1B expression was observed in OC tissues and cell lines, and it was identified as a hub DEG with prognostic value in OC. Following BUB1B knockdown, cell proliferation, migration, invasion, and tumor growth and metastasis were suppressed in\u00a0vitro and in\u00a0vivo. Mechanically, BUB1B knockdown inhibited the expression of\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin and inactivated the Wnt\/\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin pathway. In addition, BUB1B overexpression promoted the malignant behavior of OC cells, which was inhibited by LGK974.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      BUB1B is an oncogene whose expression level is negatively correlated with the prognosis of OC patients. Mechanically, BUB1B promotes the progression of OC via the Wnt\/\n                      <jats:italic>\u03b2<\/jats:italic>\n                      \u2010catenin pathway. Our study offers a potential therapeutic target for OC treatment.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.71311","type":"journal-article","created":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T05:50:55Z","timestamp":1761803455000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>BUB1B<\/scp>\n                    Promotes Ovarian Cancer Cell Proliferation and Metastasis by Activating the Wnt\/\u03b2\u2010Catenin Pathway"],"prefix":"10.1002","volume":"14","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3726-0987","authenticated-orcid":false,"given":"Jing","family":"Wang","sequence":"first","affiliation":[{"name":"The International Peace Maternity and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University  Shanghai China"},{"name":"Shanghai Key Laboratory of Embryo Original Diseases  Shanghai 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