{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T00:28:34Z","timestamp":1792801714585,"version":"4.5.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:00:00Z","timestamp":1773705600000},"content-version":"vor","delay-in-days":16,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,3,1]],"date-time":"2026-03-01T00:00:00Z","timestamp":1772323200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100009647","name":"Ministry of Health","doi-asserted-by":"publisher","award":["RC\u20102025\u20102797269"],"award-info":[{"award-number":["RC\u20102025\u20102797269"]}],"id":[{"id":"10.13039\/100009647","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The level of measurable residual disease (MRD) is one of the most important features correlating depths of response and long\u2010term outcomes in multiple myeloma (MM) and MRD evaluation is currently the gold standard tool for assessing treatment response. Nevertheless, reproducibility across laboratories is a major concern, as discrepancies among results make comparability impractical. Aims: herein, we report preliminary results from the \u201cItalian MM\u2010MRD network\u201d project.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients &amp; Methods<\/jats:title>\n                    <jats:p>MRD in bone marrow (BM) samples have been measured from newly diagnosed MM patients using next\u2010generation flow\u2010cytometry (NGF) or next\u2010generation sequencing (NGS) approaches in different laboratories.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The NGF workgroup (7 laboratories) implemented the Euro\u2010Flow Standard\u2010Operating\u2010Protocol to reach minimum 1\u2009\u00d7\u200910\n                      <jats:sup>\u22125<\/jats:sup>\n                      sensitivity. The inter\u2010operator retrospective study (Stage 1) showed high inter\u2010center concordance in monoclonal plasma cells detection (ICC\u2009=\u20090.90,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), whereas moderate concordance was observed in the inter\u2010laboratory correlation (Stage 2) in in\u2010vivo samples (ICC\u2009=\u20090.63,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), reaching a median limit\u2010of\u2010detection (LOD) and limit\u2010of\u2010quantification (LOQ) of 8\u2009\u00d7\u200910\u22126 and 2\u2009\u00d7\u200910\n                      <jats:sup>\u22125<\/jats:sup>\n                      , respectively. Greater variability was also observed in the analysis of other BM cell populations. The NGS workgroup (4 laboratories) employed a targeted amplicon\u2010based approach to detect clonotypic IGH\/IGK gene rearrangements in diagnostic samples, subsequently used to track MRD in mock samples. The experimental design was divided into three quality\u2010control (QC) rounds, focused on finding a shared strategy for clonotype identification (QC1: 100% concordance among centers), or quantifying MRD in mock samples (concordance: 81% [QC2]; 91% [QC3]). The 10\n                      <jats:sup>\u22125<\/jats:sup>\n                      \u2010sensitivity level was successfully reached in most of tested dilutions (QC2: 19\/20\u2009=\u200995%; QC3: 19\/23\u2009=\u200983%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Overall, this pilot study provided preliminary data for MRD harmonization across Italian centers, paving the way for an expanded network, aiming at reducing variability, improving comparability, and enabling broader use of MRD\u2010monitoring in clinical practice.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.71678","type":"journal-article","created":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T01:51:03Z","timestamp":1773798663000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Next Generation Flow and Next Generation Sequencing for Measurable Residual Disease Assessment in Multiple Myeloma Patients: A Real\u2010Life Italian Multicenter Harmonization Experience"],"prefix":"10.1002","volume":"15","author":[{"given":"Stefania","family":"Oliva","sequence":"first","affiliation":[{"name":"Division of Hematology, Department of Molecular Biotechnology and Health Sciences University of Torino  Turin Italy"},{"name":"Division of Hematology, A O U Citt\u00e0 Della Salute e Della Scienza di Torino University of Torino  Turin Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8196-8707","authenticated-orcid":false,"given":"Marina","family":"Martello","sequence":"additional","affiliation":[{"name":"IRCCS Azienda Ospedaliero\u2010Universitaria di Bologna, Istituto di Ematologia \u201cSer\u00e0gnoli\u201d  Bologna Italy"},{"name":"Department of Medical and Surgical Sciences University of Bologna  Bologna 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