{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T05:51:04Z","timestamp":1777009864187,"version":"3.51.4"},"reference-count":36,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T00:00:00Z","timestamp":1775174400000},"content-version":"vor","delay-in-days":2,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Cervical cytology offers a relatively safe and reliable method for cancer screening, but the tests contain vague grading criteria, such as atypical squamous cells, and cannot exclude high\u2010grade squamous intraepithelial lesions (ASC\u2010H) and atypical squamous cells of undetermined significance (ASC\u2010US).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>To explore distinct differentiation points among ASC\u2010H, high\u2010grade squamous intraepithelial lesions (HSIL), and non\u2010keratinizing squamous cell carcinoma (SCC), and among ASC\u2010US, low\u2010grade squamous intraepithelial lesions (LSIL), and negative for intraepithelial lesion or malignancy, hyperspectral imaging analyses were performed in cells mounted on 150 Papanicolaou\u2010stained specimens containing nine cervical cell types. Hyperspectral data were obtained from nuclei, cytoplasm, and whole\u2010cell regions in 4841 cells without overlap at 15\u2010nm increments in wavelength within the visible light spectrum (450\u2013750\u2009nm).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significant differences in hyperspectral intensity were observed between ASC\u2010H and HSIL at 487\u2009nm to 517\u2009nm (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.05), as well as among SCC at 457\u2009nm (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.01). Further, hyperspectral intensity differences between ASC\u2010US and LSIL were notable at multiple wavelengths with the lowest\n                      <jats:italic>p<\/jats:italic>\n                      \u2010value (\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.006) at 532\u2009nm. Interestingly, quantification of hyperspectral values revealed strong correlations between nucleus\u2010to\u2010cytoplasm ratio and hyperspectral intensity value of whole cell (\n                      <jats:italic>R<\/jats:italic>\n                      <jats:sup>2<\/jats:sup>\n                      \u2009=\u20090.8339,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), indicating that intensity from the whole cell provided the most informative indicator of cervical cells.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These results suggest that hyperspectral analysis of whole cells offers a valuable tool for differentiating atypical squamous cells from other cell types.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/cam4.71746","type":"journal-article","created":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T10:30:27Z","timestamp":1775212227000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hyperspectral Imaging of Whole\u2010Cell Region for Differentiating Cervical Squamous Intraepithelial Lesion Cytology"],"prefix":"10.1002","volume":"15","author":[{"given":"Haruka","family":"Matsukawa","sequence":"first","affiliation":[{"name":"Division of Molecular Pathology, Department of Pathological Sciences University of Fukui  Fukui Japan"},{"name":"Division of Surgical Pathology University of Fukui Hospital  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Keiko","family":"Yugawa","sequence":"additional","affiliation":[{"name":"Technology Division Panasonic Holdings Corporation  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chikai","family":"Hosokawa","sequence":"additional","affiliation":[{"name":"Technology Division Panasonic Holdings Corporation  Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumi","family":"Furuichi","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology University of Fukui Hospital  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumiko","family":"Kamada","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology University of Fukui Hospital  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoko","family":"Tanabe","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology University of Fukui Hospital  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakon","family":"Noriki","sequence":"additional","affiliation":[{"name":"Faculty of Nursing &amp; Social Welfare Sciences Fukui Prefectural University  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshiaki","family":"Imamura","sequence":"additional","affiliation":[{"name":"Division of Surgical Pathology University of Fukui Hospital  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Naiki","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology, Department of Pathological Sciences University of Fukui  Fukui Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2027-4984","authenticated-orcid":false,"given":"Kunihiro","family":"Inai","sequence":"additional","affiliation":[{"name":"Division of Molecular Pathology, Department of Pathological Sciences University of Fukui  Fukui Japan"},{"name":"Health Support Center Nagoya Institute of Technology  Aichi Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,4,3]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijgo.12611"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2023.2239085"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.1800155810"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002\u20109378(41)90932\u20106"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1159\/000328553"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.3760\/cma.j.cn112137\u201020210115\u201000134"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.25259\/CMAS_03_04_2021"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.24325"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1097\/LGT.0000000000000525"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmr.IJMR_1360_20"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1080\/13506129.2023.2253560"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201037766\u20104"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010023\u201046090\u20103"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jhbp.798"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11548\u2010024\u201003061\u2010x"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41566\u2010022\u201001133\u20101"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.3390\/s20071911"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.6335"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.6320"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1364\/BOE.396882"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1159\/000477556"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.33566"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.2307\/4615733"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00428\u2010014\u20101597\u20108"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.25259\/CMAS_04_04_2021"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-11074-5"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2019.103344"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1002\/dc.2840040404"},{"key":"e_1_2_11_31_1","first-page":"15","volume-title":"Nuclear\/Cytoplasmic Ratio (N\/C) and Nuclear Chromatin. 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