{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T05:49:16Z","timestamp":1777009756095,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T00:00:00Z","timestamp":1775606400000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"},{"start":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T00:00:00Z","timestamp":1775001600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["JP23725350"],"award-info":[{"award-number":["JP23725350"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["18007279"],"award-info":[{"award-number":["18007279"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Transgelin is an actin\u2010binding protein that promotes cancer progression via activation of cancer\u2010associated fibroblasts and has been identified as a prognostic marker. However, its distribution and functional role in colon cancer remain unclear. In this study, we aimed to elucidate the mechanistic role of transgelin in colon cancer progression by focusing on its functional impact in cancer\u2010associated fibroblasts. Tissue microarrays from 359 human colon cancer tissues were investigated to elucidate the clinical importance of transgelin expression in cancer stroma. We focused on transgelin in fibroblasts and investigated its functional role in stromal activation using in\u00a0vitro knockdown experiments and in\u00a0vivo co\u2010transplantation models. Primary cultures of human colon fibroblasts were evaluated for their biological function. Our data showed that transgelin expression is predominant in activated cancer\u2010associated fibroblasts in colon cancer tissues. Stimulation by cancer\u2010cell\u2010conditioned medium (CM) significantly upregulated transgelin, ACTA2, COL1A1, and TNC expression in colonic fibroblasts. Additionally, transgelin knockdown (KD) in fibroblasts did not influence the upregulation except for transgelin itself. Transgelin KD in fibroblasts did not result in drastic alterations in gene expression profiles. Transgelin KD suppressed collagen gel contractility. Furthermore, co\u2010transplantation experiments of cancer cells and colonic fibroblasts into immunodeficient mice revealed that transgelin KD inhibited tumor growth in fibroblasts. In conclusion, stromal transgelin expression in colon cancer strongly correlated with distant metastasis and served as a prognostic factor for colon cancer.<\/jats:p>\n                  <jats:p>Mechanistically, transgelin in cancer\u2010associated fibroblasts promotes tumor growth by regulating stromal contractility, suggesting transgelin as a potential therapeutic target.<\/jats:p>","DOI":"10.1002\/cam4.71789","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T05:25:40Z","timestamp":1775712340000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Transgelin Expression in Activated Cancer\u2010Associated Fibroblasts Regulates Stromal Contractility and Promotes Colon Cancer Progression"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3422-9676","authenticated-orcid":false,"given":"Ryuji","family":"Okamoto","sequence":"first","affiliation":[{"name":"Department of Pathology and Clinical Laboratories National Cancer Center Hospital East  Kashiwa Japan"},{"name":"Department of Gastroenterology and Endoscopy National Cancer Center Hospital East  Kashiwa Japan"},{"name":"Course of Advanced Clinical Research Cancer Juntendo University Graduate School of Medicine  Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuro","family":"Hiroi","sequence":"additional","affiliation":[{"name":"Department of Pathology and Clinical Laboratories National Cancer Center Hospital East  Kashiwa Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yuka","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Division of Innovative Pathology and Laboratory Medicine National Cancer Center 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