{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:54:24Z","timestamp":1781585664452,"version":"3.54.5"},"reference-count":37,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T00:00:00Z","timestamp":1776988800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["21K16428"],"award-info":[{"award-number":["21K16428"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["23K15480"],"award-info":[{"award-number":["23K15480"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007449","name":"Takeda Science Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007449","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007533","name":"Kobayashi Foundation for Cancer Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100007533","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008673","name":"Yasuda Memorial Medical Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008673","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100008732","name":"Uehara Memorial Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100008732","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008880","name":"Kanae Foundation for the Promotion of Medical Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100008880","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005865","name":"Mochida Memorial Foundation for Medical and Pharmaceutical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["S250264"],"award-info":[{"award-number":["S250264"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Colorectal cancer (CRC) with peritoneal dissemination remains a major therapeutic challenge because of poor prognosis and limited treatment options. Experimental models that accurately recapitulate tumor\u2013mesothelial interactions are scarce. Here, we report the establishment of a novel autologous paired model comprising a CRC cell line (OMUCR\u20101) and matched cancer\u2010associated mesothelial cells (CAmeso), both simultaneously derived from the malignant ascites of the same patient. Lineage marker analysis using qPCR demonstrated that OMUCR\u20101 selectively expressed epithelial markers (\n                    <jats:italic>EPCAM, KRT20<\/jats:italic>\n                    ), whereas CAmeso strongly expressed mesothelial\u2010mesenchymal markers (ACTA2, MSLN) and lacked epithelial marker expression. These mutually exclusive expression patterns confirm that the two cell populations are phenotypically distinct and rule out cross\u2010contamination. OMUCR\u20101 displayed strong tumorigenic capacity across multiple transplantation models. CAmeso enhanced CRC cell migration and invasion in\u00a0vitro, and co\u2010transplantation with OMUCR\u20101 resulted in larger tumors enriched with \u03b1SMA\u2010positive stromal components. RNA sequencing of co\u2010injected xenografts revealed increased expression of murine stromal Fgfr3. Treatment with the FGFR inhibitor BGJ398 reduced tumor growth and decreased stromal FGFR3\u2010positive components, suggesting that stromal FGFR3 may represent a potential microenvironmental vulnerability in CRC with peritoneal dissemination. This autologous CRC\u2010mesothelial system provides a physiologically relevant platform for dissecting tumor\u2010stroma interactions in peritoneal metastasis and may advance stromal\u2010targeted therapeutic strategies.\n                  <\/jats:p>","DOI":"10.1002\/cam4.71804","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T04:29:48Z","timestamp":1777091388000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Simultaneous Establishment of Autologous Colorectal Cancer and Mesothelial Stromal Cell Lines from Malignant Ascites Reveals a Mesothelial\u2010Stromal\n                    <scp>FGFR3<\/scp>\n                    Axis as a Potential Vulnerability in Peritoneal Metastasis"],"prefix":"10.1002","volume":"15","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8878-883X","authenticated-orcid":false,"given":"Yasuhiro","family":"Fukui","sequence":"first","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5234-4030","authenticated-orcid":false,"given":"Hiroaki","family":"Kasashima","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zizhou","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iguru","family":"Omori","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yukina","family":"Kusunoki","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanae","family":"Echizen","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiki","family":"Nonaka","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Seki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kuroda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0116-0922","authenticated-orcid":false,"given":"Yuichiro","family":"Miki","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mami","family":"Yoshii","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsunari","family":"Fukuoka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatsuro","family":"Tamura","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masatsune","family":"Shibutani","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takahiro","family":"Toyokawa","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Muta","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyoto University, Graduate School of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Nakanishi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Kyoto University, Graduate School of Medicine  Kyoto Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Yashiro","sequence":"additional","affiliation":[{"name":"Molecular Oncology and Therapeutics Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Ohtani","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiyoshi","family":"Maeda","sequence":"additional","affiliation":[{"name":"Department of Gastroenterological Surgery Osaka Metropolitan University, Graduate School of Medicine  Osaka Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,24]]},"reference":[{"key":"e_1_2_13_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21395"},{"key":"e_1_2_13_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.sla.0000197702.46394.16"},{"key":"e_1_2_13_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/cas.70021"},{"key":"e_1_2_13_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers13050963"},{"key":"e_1_2_13_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11252"},{"key":"e_1_2_13_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2017.12.004"},{"key":"e_1_2_13_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07747-9"},{"key":"e_1_2_13_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-3212"},{"key":"e_1_2_13_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-018-0180-x"},{"key":"e_1_2_13_11_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers12020499"},{"key":"e_1_2_13_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2025.217686"},{"key":"e_1_2_13_13_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-2-23"},{"key":"e_1_2_13_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.4281"},{"key":"e_1_2_13_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27931"},{"key":"e_1_2_13_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2021.100297"},{"key":"e_1_2_13_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2020.10.014"},{"key":"e_1_2_13_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24763"},{"key":"e_1_2_13_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.5403"},{"key":"e_1_2_13_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.08.014"},{"key":"e_1_2_13_21_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0178635"},{"key":"e_1_2_13_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2015.236"},{"key":"e_1_2_13_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-004-5768-5"},{"key":"e_1_2_13_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2023.113613"},{"issue":"656","key":"e_1_2_13_25_1","article-title":"Mesothelial Cell\u2010Derived Antigen\u2010Presenting Cancer\u2010Associated Fibroblasts Induce Expansion of Regulatory T Cells in Pancreatic Cancer","volume":"40","author":"Huang H.","year":"2022","journal-title":"Cancer Cell"},{"key":"e_1_2_13_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-017-0691-y"},{"key":"e_1_2_13_27_1","doi-asserted-by":"publisher","DOI":"10.3892\/ijo.2016.3829"},{"key":"e_1_2_13_28_1","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.163019"},{"key":"e_1_2_13_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0086516"},{"key":"e_1_2_13_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2023.1244345"},{"key":"e_1_2_13_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/35065016"},{"key":"e_1_2_13_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2012.08.013"},{"key":"e_1_2_13_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208927"},{"key":"e_1_2_13_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature03688"},{"key":"e_1_2_13_35_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20431"},{"key":"e_1_2_13_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc745"},{"key":"e_1_2_13_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2014.05.004"},{"key":"e_1_2_13_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2025.117221"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.71804","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.71804","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.71804","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,16]],"date-time":"2026-06-16T04:19:57Z","timestamp":1781583597000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.71804"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,24]]},"references-count":37,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2026,5]]}},"alternative-id":["10.1002\/cam4.71804"],"URL":"https:\/\/doi.org\/10.1002\/cam4.71804","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,4,24]]},"assertion":[{"value":"2025-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-03-31","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-24","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71804"}}