{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T20:13:20Z","timestamp":1792181600424,"version":"4.4.0"},"reference-count":22,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T00:00:00Z","timestamp":1780272000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82503839"],"award-info":[{"award-number":["82503839"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Cancer Medicine"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>Breast cancer, now the fourth leading cause of cancer\u2010related mortality worldwide, necessitates early detection for improved clinical outcomes. Conventional histopathology, though widely used, is invasive and subjective, limiting its utility in early\u2010stage diagnosis. Here, we integrated confocal Raman spectroscopy with metabolomics to analyze biochemical and morphological features of breast tissues, including normal, fibroadenoma, ductal carcinoma in\u00a0situ (DCIS), and invasive ductal carcinoma (IDC). Using spectral analysis and spectral unmixing, we mapped key biochemical components\u2014proteins, lipids, and nucleic acids\u2014across distinct tissue types. Cancerous tissues displayed heightened signals for proteins and nucleic acids but reduced lipids and carotenoids, reflecting profound metabolic alterations. Validation through metabolomic profiling revealed upregulated glycolytic and lipid synthesis pathways in tumor regions. Raman imaging further enabled precise classification of breast cancer subtypes, such as mucinous carcinoma and phyllodes tumors. These findings underscore the potential of Raman spectroscopy as a non\u2010invasive diagnostic modality for early detection and classification of breast cancer. The integration of Raman imaging with machine learning presents a promising avenue for advancing precision oncology.<\/jats:p>","DOI":"10.1002\/cam4.71861","type":"journal-article","created":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T05:17:14Z","timestamp":1780377434000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Integration of Raman Spectroscopy and Metabolomics for Early Breast Cancer Detection and Classification"],"prefix":"10.1002","volume":"15","author":[{"given":"Xue","family":"Li","sequence":"first","affiliation":[{"name":"Department of Breast Surgery Harbin Medical University Cancer Hospital  Harbin Heilongjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9266-3834","authenticated-orcid":false,"given":"He","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Harbin Medical University Cancer Hospital  Harbin Heilongjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhan","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Breast Surgery Harbin Medical University Cancer Hospital  Harbin Heilongjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0677-3245","authenticated-orcid":false,"given":"Yang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pharmacy Harbin Medical University  Harbin Heilongjiang P.R. China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangjie","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Beijing Chaoyang Hospital Capital Medical University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,6,1]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21834"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamaoncol.2023.4837"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2022.40.16_suppl.e24036"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.p1355"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2020.03.014"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a041647"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/03000605241259682"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.aaw5473"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.5c00023"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/wnan.70015"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2022.03.036"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bios.2022.114075"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ctm2.1345"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.microc.2023.108900"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2021.120300"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.74002"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/alz.074882"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.actbio.2024.11.002"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2021.119732"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-85309-z"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2018.36.15_suppl.11554"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-025-01274-1"}],"container-title":["Cancer Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.71861","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/cam4.71861","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/cam4.71861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T10:05:27Z","timestamp":1784196327000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/cam4.71861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6]]},"references-count":22,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2026,6]]}},"alternative-id":["10.1002\/cam4.71861"],"URL":"https:\/\/doi.org\/10.1002\/cam4.71861","archive":["Portico"],"relation":{},"ISSN":["2045-7634","2045-7634"],"issn-type":[{"value":"2045-7634","type":"print"},{"value":"2045-7634","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6]]},"assertion":[{"value":"2025-10-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-04-13","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-06-01","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e71861"}}